Leptin Antikörper

Produktnummer ABIN5706823

Hersteller Produkt- Nr.: 110-401-320-0100

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch Leptin in WB und ELISA. Er zeigt eine Reaktivität gegenüber Human und Maus.

Kurzübersicht für Leptin Antikörper (ABIN5706823)

Target: Leptin (LEP)

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Leptin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Produktdetails anti-Leptin Antikörper

Hersteller Produkt- Nr.: 110-401-320-0100

Hersteller: Rockland

Verwendungszweck: Leptin Antibody

Kreuzreaktivität (Details): The antibody will recognize recombinant and native 16 kDa leptin from mouse and human.

Produktmerkmale: Synonyms: rabbit anti-Leptin, LEP antibody, Leptin Murine Obesity Homolog antibody, Leptin Precursor Obesity Factor antibody, OB antibody, Obese Protein antibody

Aufreinigung: This antiserum has been heated to 56°C for 30 minutes.

Sterilität: Sterile filtered

Immunogen:

Immunogen: This whole rabbit serum was prepared by repeated immunizations with recombinant mouse leptin 16,000 MW produced in E. coli.

Immunogen Type: Recombinant Protein

Anwendungsinformationen

Applikationshinweise:

Application Note: This antiserum against Mouse Leptin has been tested for use in ELISA and immunoblotting.  Reactivity in other immunoassays is unknown.  This product has been assayed by immunoblot against tissue homogenates using HRP Goat-anti-Rabbit IgG [H&L] (code # 611-1302) and TMB as a substrate.  A working dilution range of 1:200 to 1:400 is suggested for this application. This product has been assayed by ELISA against recombinant mouse leptin using HRP Conjugated Anti-Rabbit IgG [H&L] (Goat) (code # 611-1302) and ABTS as a substrate for 30 minutes at room temperature.  A working dilution of 1:2,400 is suggested for this product.

Western Blot Dilution: 1:500 - 1:2,000

ELISA Dilution: 1:1,000 - 1:5,000

Other: User Optimized

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer:

Buffer: None

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Haltbarkeit: 12 months

Details zu Leptin

Target: Leptin (LEP)

Andere Bezeichnung: Lep

Hintergrund: Background: Leptin is a key player in the regulation of energy balance and body weight control. Obesity associated leptin is the product of the OB gene and has been identified with Type II diabetes. Once released into the circulation, it has central and peripheral effects by binding LEPR, found in many tissues, which results in the activation of several major signaling pathways. It acts as an appetite-regulating factor, regulates bone mass and secretion of hypothalamo-pituitary-adrenal hormones, it increases basal metabolism, influences reproductive function, regulates pancreatic beta-cell function and insulin secretion, it induces FOS and SOCS3 expression to release anorexigenic peptides. It has a modulatory role in nutrient absorption. It reduces glucose absorption by enterocytes by activating PKC and leading to a sequential activation of p38, PI3K and ERK signaling pathways which exerts an inhibitory effect on glucose absorption. It acts as a growth factor on certain tissues, through the activation of different signaling pathways increases expression of genes involved in cell cycle regulation such as CCND1, via JAK2-STAT3 pathway, or VEGFA, via MAPK1/3 and PI3K-AKT1 pathways. It may also play an apoptotic role via JAK2-STAT3 pathway and up-regulation of BIRC5 expression. It plays a pro-inflammatory role, in synergy with IL1B, by inducing NOS2 which promotes the production of IL6, IL8 and Prostaglandin E2, through a signaling pathway that involves JAK2, PI3K, MAP2K1/MEK1 and MAPK14/p38. In adaptive immunity, promotes the switch of memory T-cells towards T helper-1 cell immune responses. Increases CD4+CD25- T cells proliferation and reduces autophagy during TCR (T cell receptor) stimulation, through MTOR signaling pathway activation and BCL2 up-regulation.

Gen-ID: 16846

UniProt: P41160

Pathways: JAK-STAT Signalweg, AMPK Signaling, Hormone Transport, Peptide Hormone Metabolism, Hormone Activity, Negative Regulation of Hormone Secretion, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Monocarboxylic Acid Catabolic Process