STAT1 Antikörper

Produktnummer ABIN5698431

Dieser Anti-STAT1 Antikörper ist ein Kaninchen Polyklonal Antikörper zur Detektion von STAT1 in WB, IHC, ELISA und FACS. Geeignet für Human, Maus und Ratte.

Kurzübersicht für STAT1 Antikörper (ABIN5698431)

Target: STAT1 (Signal Transducer and Activator of Transcription 1, 91kDa (STAT1))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser STAT1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS)

Produktdetails anti-STAT1 Antikörper

Verwendungszweck: STAT1 antibody

Immunogen: signal transducer and activator of transcription 1, 91kDa

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

PC-3 cells were subjected to SDS PAGE followed by western blot with FNab08295(STAT1 antibody) at dilution of 1:600

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol  pH 7.3

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freeze / thaw cycles.

Lagerung: -20 °C

Informationen zur Lagerung: -20°C for 12 months

Haltbarkeit: 12 months

Details zu STAT1

Target: STAT1 (Signal Transducer and Activator of Transcription 1, 91kDa (STAT1))

Andere Bezeichnung: STAT1

Hintergrund:

Synonyms: Signal transducer and activator of transcription 1-alpha/beta|Transcription factor ISGF-3 components p91/p84|STAT1

Background: Signal transducer and transcription activator that mediates cellular responses to interferons(IFNs), cytokine KITLG/SCF and other cytokines and other growth factors. Following type I IFN(IFN-alpha and IFN-beta) binding to cell surface receptors, signaling via protein kinases leads to activation of Jak kinases(TYK2 and JAK1) and to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize and associate with ISGF3G/IRF-9 to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element(ISRE) to activate the transcription of IFN-stimulated genes(ISG), which drive the cell in an antiviral state. In response to type II IFN(IFN-gamma), STAT1 is tyrosine-and serine-phosphorylated. It then forms a homodimer termed IFN-gamma-activated factor(GAF), migrates into the nucleus and binds to the IFN gamma activated sequence(GAS) to drive the expression of the target genes, inducing a cellular antiviral state. Becomes activated in response to KITLG/SCF and KIT signaling. May mediate cellular responses to activated FGFR1, FGFR2, FGFR3 and FGFR4.

Gen-ID: 6772

UniProt: P42224

Pathways: JAK-STAT Signalweg, RTK Signalweg, Interferon-gamma Pathway, Response to Growth Hormone Stimulus, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Endopeptidase Activity, Hepatitis C, CXCR4-mediated Signaling Events