Prothrombin Antikörper (AA 67-124)

Produktnummer ABIN5693261

Produktdetails anti-Prothrombin Antikörper

Target: Prothrombin (F2) (Coagulation Factor II (thrombin) (F2))

Bindungsspezifität: AA 67-124

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Prothrombin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunocytochemistry (ICC), Flow Cytometry (FACS)

Verwendungszweck: Anti-Prothrombin/F2 Antibody Picoband®

Marke: Picoband™

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Rabbit IgG polyclonal antibody for Prothrombin detection. Tested with WB, IHC-P, Direct ELISA in Human.

Aufreinigung: Immunogen affinity purified.

Immunogen: E. coli-derived human Prothrombin recombinant protein (Position: Y67-R124).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.1-0.5 μg/mL
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL
Immunohistochemistry (Frozen Section), 0.5-1 μg/mL
Immunocytochemistry, 0.5-1 μg/mL
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells
ELISA, 0.1-0.5 μg/mL
1. Lancellotti, S., De Cristofaro, R. Congenital prothrombin deficiency. Semin. Thromb. Hemost. 35: 367-381, 2009. 2. Royle, N. J., Irwin, D. M., Koschinsky, M. L., MacGillivray, R. T. A., Hamerton, J. L. Human genes encoding prothrombin and ceruloplasmin map to 11p11-q12 and 3q21-24, respectively. Somat. Cell Molec. Genet. 13: 285-292, 1987.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na₂HPO₄, 0.05 mg NaN₃.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Referenzen für anti-Prothrombin Antikörper (ABIN5693261)

Shen, Fang, Lv, Pei, Wang, Jiang, Ding: "Heparin impairs angiogenesis through inhibition of microRNA-10b." in: The Journal of biological chemistry, Vol. 286, Issue 30, pp. 26616-27, (2011) (PubMed).

Details zu Prothrombin

Target: Prothrombin (F2) (Coagulation Factor II (thrombin) (F2))

Andere Bezeichnung: F2 (F2 Produkte)

Hintergrund:

Synonyms: Prothrombin, Coagulation factor II, Activation peptide fragment 1, Activation peptide fragment 2, Thrombin light chain, Thrombin heavy chain, F2

Tissue Specificity: Expressed by the liver and secreted in plasma.

Background: F2 (Coagulation Factor II), also known as thrombin, is a serine protease that in humans is encoded by the F2 gene. This gene for human prothrombin (F2) was assigned to chromosome 11p11-q12 by analysis of a panel of somatic cell hybrid DNAs and by in situ hybridization, using both cDNA and genomic probes. The activated thrombin enzyme plays an important role in hemostasis and thrombosis: it converts fibrinogen to fibrin for blood clot formation, stimulates platelet aggregation, and activates coagulation factors V, VIII (F8), and XIII (F13A1). Thrombin also inhibits coagulation by activating protein C.

Molekulargewicht: 85 kDa

UniProt: P00734

Pathways: Komplementsystem, Peptide Hormone Metabolism, Regulation of G-Protein Coupled Receptor Protein Signaling