CHEK1 Antikörper (AA 315-319)

Produktnummer ABIN5689595

Dieses Anti-CHEK1-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von CHEK1 in WB und IHC. Geeignet für Human und Maus.

Kurzübersicht für CHEK1 Antikörper (AA 315-319) (ABIN5689595)

Target: CHEK1 (Checkpoint Kinase 1 (CHEK1))

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CHEK1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Produktdetails anti-CHEK1 Antikörper

Bindungsspezifität: AA 315-319

Spezifität: This antibody detects endogenous level of total Chk1 protein.

Aufreinigung: Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Immunogen: Chk1 (Ab-317) antibody was raised against a peptide sequence around aa.315~319 (S-S-S-Q-P) derived from Human Chk1.

Anwendungsinformationen

Applikationshinweise: Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+),  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store antibody at -20°C for up to one year.

Details zu CHEK1

Target: CHEK1 (Checkpoint Kinase 1 (CHEK1))

Andere Bezeichnung: Chk1

Hintergrund: Chek1 is a protein kinase that inhibits mitotic entry after DNA damage, required for the DNA damage checkpoint and is strongly similar to murine Chek1. Checkpoint pathways control the order and timing of cell cycle transitions and ensure that critical events, such as DNA replication and chromosome segregation, are completed with high fidelity. The mouse and human proteins share 90 % sequence identity through the protein kinase domains. The sequence of the 476-amino acid human Chek1 protein is 29 %, 40 %, and 44 % identical to those of the fission yeast Chek1, C. elegans Chek1, and Drosophila 'grapes' (Grp) proteins, respectively. Chek1 is expressed ubiquitously as an approximately 2.4-kb mRNA, with the most abundant expression in thymus, testis, small intestine, and colon. The protein has altered mobility when isolated from cells treated with ionizing radiation, indicating that Chek1 is modified in response to DNA damage. In vitro, Chek1 directly phosphorylates a regulator of CDC2 tyrosine phosphorylation, CDC25C. In response to DNA damage, Chek1 phosphorylates and inhibits CDC25C, thus preventing activation of the CDC2-Cyclin-B complex and mitotic entry

Molekulargewicht: 56 kDa

Gen-ID: 1111

NCBI Accession: NP_001107593

UniProt: O14757

Pathways: p53 Signalweg, Apoptose, Zellzyklus, DNA Reparatur