LIN28B Antikörper (AA 242-246)

Produktnummer ABIN5689573

Der Kaninchen Polyklonal Anti-LIN28B-Antikörper wurde für WB validiert. Er ist geeignet, LIN28B in Proben von Human zu detektieren.

Kurzübersicht für LIN28B Antikörper (AA 242-246) (ABIN5689573)

Target: LIN28B (Lin-28 Homolog B (LIN28B))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser LIN28B Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-LIN28B Antikörper

Bindungsspezifität: AA 242-246

Spezifität: This antibody detects endogenous levels of total LIN28B protein.

Aufreinigung: Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Immunogen: LIN28B antibody was raised against a peptide sequence around aa.242~246 ( P-S-V-Q-K) derived from Human LIN28B.

Anwendungsinformationen

Applikationshinweise: Western Blot: 1:500~1:1000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+),  pH 7.4, 150  mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store antibody at -20°C for up to one year.

Details zu LIN28B

Target: LIN28B (Lin-28 Homolog B (LIN28B))

Andere Bezeichnung: LIN28B

Hintergrund: Acts as a suppressor of microRNA (miRNA) biogenesis by specifically binding the precursor let-7 (pre-let-7), a miRNA precursor. Acts by binding pre-let-7 and recruiting ZCCHC11/TUT4 uridylyltransferase, leading to the terminal uridylation of pre-let-7. Uridylated pre-let-7 miRNAs fail to be processed by Dicer and undergo degradation. Specifically recognizes the 5'-GGAG-3' motif in the terminal loop of pre-let-7. Also recognizes and binds non pre-let-7 pre-miRNAs that contain the 5'-GGAG-3' motif in the terminal loop, leading to their terminal uridylation and subsequent degradation. Mediates MYC-mediated let-7 repression. Isoform 1, when overexpressed, stimulates growth of the breast adenocarcinoma cell line MCF-7. Isoform 2 has no effect on cell growth.

Molekulargewicht: 21 32 kDa

Gen-ID: 389421

NCBI Accession: NP_001004317

UniProt: Q6ZN17