IL1A Antikörper

Produktnummer ABIN5689137

Dieser Kaninchen Polyklonal Antikörper erkennt spezifisch IL1A in WB, ELISA und Neut. Er zeigt eine Reaktivität gegenüber Maus.

Kurzübersicht für IL1A Antikörper (ABIN5689137)

Target: IL1A (Interleukin 1 alpha (IL1A))

Reaktivität: Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser IL1A Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Neutralization (Neut)

Produktdetails anti-IL1A Antikörper

Aufreinigung: Anti-mIL-1 alpha specific antibody was purified by affinity chromatography employing immobilized mIL-1 alpha matrix.

Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant mIL-1-alpha (murine IL-1 alpha).

Anwendungsinformationen

Applikationshinweise: Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of mIL-1 alpha (0.05 ng/mL), a concentration of 0.03 - 0.05 μ,g/mL of this antibody is required.

ELISA:

To detect mIL-1 alpha by direct ELISA (using 100 μ,L/well antibody solution) a concentration of at least 0.5 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant mIL-1 alpha.

Sandwich:

To detect mIL-1α, by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.5 - 2.0 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Murine IL-1α, (XP-5175Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mIL-1α,.

Western Blot:

To detect mIL-1 alpha by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-1 alpha is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Lagerung: -20 °C

Informationen zur Lagerung: IL-1a antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.

Details zu IL1A

Target: IL1A (Interleukin 1 alpha (IL1A))

Andere Bezeichnung: IL-1a

Gen-ID: 16175

UniProt: P01582

Pathways: NF-kappaB Signalweg, Autophagie, Cancer Immune Checkpoints