TRAIL Antikörper

Produktnummer ABIN5689123

Der Kaninchen Polyklonal Anti-TRAIL-Antikörper wurde für WB, ELISA und Neut validiert. Er ist geeignet, TRAIL in Proben von Human zu detektieren.

Kurzübersicht für TRAIL Antikörper (ABIN5689123)

Target: TRAIL (TNFSF10) (Tumor Necrosis Factor (Ligand) Superfamily, Member 10 (TNFSF10))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser TRAIL Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Neutralization (Neut)

Produktdetails anti-TRAIL Antikörper

Aufreinigung: Anti-hTRAIL/Apo2L specific antibody was purified by affinity chromatography employing immobilized hTRAIL/Apo2L matrix.

Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hTRAIL/Apo2L (humanTRAIL/Apo2L).

Anwendungsinformationen

Applikationshinweise: Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of hTRAIL/Apo2L (30 ng/mL), a concentration of 0.5 - 0.8 μ,g/mL of this antibody is required.

ELISA:

To detect hTRAIL/Apo2L by direct ELISA (using 100 μ,L/well antibody solution) a concentration of at least 0.5 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hTRAIL/Apo2L.

Sandwich:

To detect hTRAIL/Apo2L by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.5 - 2.0 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human TRAIL/Apo2L (XP-5289Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hTRAIL/Apo2L.

Western Blot:

To detect hTRAIL/Apo2L by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hTRAIL/Apo2L is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Lagerung: -20 °C

Informationen zur Lagerung: TRAIL/Apo2L antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.

Details zu TRAIL

Target: TRAIL (TNFSF10) (Tumor Necrosis Factor (Ligand) Superfamily, Member 10 (TNFSF10))

Andere Bezeichnung: TRAIL

Hintergrund: Apoptosis or programmed cell death is induced in cells by a group of death domain containing receptors. Binding of ligand to these receptors sends signals that activate members of the caspase family of proteases. The signals ultimately cause degradation of chromosomal DNA by activating DNase. TRAIL (TNF related apoptosis induced ligand) or Apo 2L initiates apoptosis of tumor cells by binding to either of its receptors, DR4 or DR5. These receptors consist of an extracellular TRAIL binding domain and a cytoplasmic "death domain".

Gen-ID: 8743

UniProt: P50591

Pathways: Apoptose, Positive Regulation of Endopeptidase Activity