EGF Antikörper

Produktnummer ABIN5688983

Dieses Anti-EGF-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von EGF in WB, ELISA und Neut. Geeignet für Human.

Kurzübersicht für EGF Antikörper (ABIN5688983)

Target: EGF (Epidermal Growth Factor (EGF))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser EGF Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Neutralization (Neut)

Produktdetails anti-EGF Antikörper

Aufreinigung: Anti-hEGF specific antibody was purified by affinity chromatography employing immobilized hEGF matrix.

Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hEGF.

Anwendungsinformationen

Applikationshinweise: Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of hEGF (2.0 ng/mL), a concentration of 0.25 0.35 μ,g/mL of this antibody is required.

ELISA:

To detect hEGF by direct ELISA (using 100us antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hEGF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Sandwich: To detect hEGF by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.5 - 2.0 μ,g/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human EGF (XP-5119Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hEGF.

Western Blot:
To detect hEGF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hEGF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Lagerung: -20 °C

Informationen zur Lagerung: EGF antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.

Details zu EGF

Target: EGF (Epidermal Growth Factor (EGF))

Andere Bezeichnung: EGF

Molekulargewicht: 6.2 kDa

Gen-ID: 1950

UniProt: P01133

Pathways: NF-kappaB Signalweg, RTK Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation