Leptin Antikörper

Produktnummer ABIN5688720

Dieses Maus Monoklonal-Antikörper erkennt spezifisch Leptin in WB, ELISA und Neut. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für Leptin Antikörper (ABIN5688720)

Target: Leptin (LEP)

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Leptin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Neutralization (Neut)

Klon: G1_4B6-3

Produktdetails anti-Leptin Antikörper

Aufreinigung: This IgG1K antibody was purified from cell culture by Protein A affinity chromatography.

Immunogen: Leptin antibody is produced in BALB/c mice using highly pure (>98%) recombinant human Leptin as the immunizing antigen.

Anwendungsinformationen

Applikationshinweise: Neutralization:
To yield one-half maximal inhibition [ND50] of the biological activity of mIL-2 (1.0 ng/mL), a concentration of 10.0-14.0 μ,g/mL of this antibody is required. ELISA:

In a sandwich ELISA (assuming 100 μ,L/well), a concentration of 4.0-8.0 μ,g/mL of this antibody will detect at least 32 pg/mL of recombinant murine IL-2 when used with our biotinylated antigen affinity purified anti-murine IL-2 (XP-5183Bt) as the detection antibody at a concentration of at least 1.0-2.0 μ,g/mL.

Western Blot:

To detect mIL-2 by Western Blot analysis this antibody can be used at a concentration of 2.0-4.0 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-2 is 1.0-2.0 ng/lane, under reducing conditions and 0.2-0.4 ng/lane, under non-reducing conditions.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Leptin lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. Reconstitute in sterile water to a concentration of 0.1 - 1.0 mg/mL. The antibody is stable for at least six weeks at 2-4°C when reconstituted.

Details zu Leptin

Target: Leptin (LEP)

Andere Bezeichnung: Leptin

Hintergrund: Leptin plays a critical role in the regulation of body weight by inhibiting food intake and stimulating energy expenditure. Defects in Leptin production cause severe hereditary obesity in rodents and humans. In addition to its effects on body weight, leptin has a variety of other functions, including the regulation of hematopoiesis, angiogenesis, wound healing, and the immune and inflammatory response. The Leptin gene is the human homolog of the gene (ob) mutant in the mouse 'obese' phenotype.

Was product number XP-5712-M

Gen-ID: 3952

UniProt: P41159

Pathways: JAK-STAT Signalweg, AMPK Signaling, Hormone Transport, Peptide Hormone Metabolism, Hormone Activity, Negative Regulation of Hormone Secretion, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Monocarboxylic Acid Catabolic Process