IGF1 Antikörper (Biotin)

Produktnummer ABIN5687628

Dieses Ziege Polyklonal-Antikörper erkennt spezifisch IGF1 in WB und ELISA. Er zeigt eine Reaktivität gegenüber Maus.

Kurzübersicht für IGF1 Antikörper (Biotin) (ABIN5687628)

Target: IGF1 (Insulin-Like Growth Factor 1 (IGF1))

Reaktivität: Maus

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser IGF1 Antikörper ist konjugiert mit Biotin

Applikation: Western Blotting (WB), ELISA

Produktdetails anti-IGF1 Antikörper (Biotin)

Aufreinigung: Anti-mIGF-I specific antibody was purified by affinity chromatography and then biotinylated.

Immunogen: Produced from sera of goats pre-immunized with highly pure (>98%) recombinant mIGF-I (murine IGF-I).

Anwendungsinformationen

Applikationshinweise: ELISA:

To detect mIGF-I by direct ELISA (using 100 μ,L/well antibody solution) this antibody can be used at a concentration of 0.15 - 0.30 μ,g/mL. Used in conjunction with compatible secondary reagents, allows the detection of at least 0.2 ng/well of recombinant mIGF-I.

Sandwich

To detect mIGF-I by sandwich ELISA (using 100 μ,L/well antibody solution) a concentration of 0.25 - 1.0 μ,g/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our Polyclonal Anti-Murine IGF-I (XP-5159) as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mIGF-I.

Western Blot:

To detect mIGF-I by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIGF-I is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Lagerung: -20 °C

Informationen zur Lagerung: IGF-I antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.

Details zu IGF1

Target: IGF1 (Insulin-Like Growth Factor 1 (IGF1))

Andere Bezeichnung: IGF-I

Gen-ID: 16000

UniProt: P05017

Pathways: RTK Signalweg, Intracellular Steroid Hormone Receptor Signaling Pathway, Peptide Hormone Metabolism, Hormone Activity, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Stem Cell Maintenance, Glycosaminoglycan Metabolic Process, Regulation of Carbohydrate Metabolic Process, Autophagie, Smooth Muscle Cell Migration, Activated T Cell Proliferation, Positive Regulation of fat Cell Differentiation