EGF Antikörper (Biotin)

Produktnummer ABIN5687626

Der Ziege Polyklonal anti-EGF Antikörper wird verwendet zum Nachweis von EGF in Proben von Maus. Er wurde validiert für WB, ELISA und Neut.

Kurzübersicht für EGF Antikörper (Biotin) (ABIN5687626)

Target: EGF (Epidermal Growth Factor (EGF))

Reaktivität: Maus

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser EGF Antikörper ist konjugiert mit Biotin

Applikation: Western Blotting (WB), ELISA, Neutralization (Neut)

Produktdetails anti-EGF Antikörper (Biotin)

Aufreinigung: Anti-mEGF specific antibody was purified by affinity chromatography and then biotinylated.

Immunogen: Produced from sera of goats pre-immunized with highly pure (>98%) recombinant mEGF (murine EGF).

Anwendungsinformationen

Applikationshinweise: Neutralization:

To yield one-half maximal inhibition [ND50] of the biological activity of Murine EGF (0.1 ng/mL), a concentration of 3.0-5.0 ng/mL of this antibody is required.ELISA:

To detect mEGF by direct ELISA (using 100 μ,L/well antibody solution) this antibody can be used at a concentration of 0.15 - 0.30 μ,g/mL. Used in conjunction with compatible secondary reagents, allows the detection of at least 0.2 ng/well of recombinant mEGF.

Western Blot:
To detect mEGF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 μ,g/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mEGF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Lagerung: -20 °C

Informationen zur Lagerung: EGF antibody is stable for at least 2 years from date of receipt at -20°C. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C. Avoid repeated freeze-thaw cycles.

Details zu EGF

Target: EGF (Epidermal Growth Factor (EGF))

Andere Bezeichnung: EGF

Gen-ID: 13645

UniProt: P01132

Pathways: NF-kappaB Signalweg, RTK Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation