LDHB Antikörper (Biotin)

Produktnummer ABIN568275

Dieses Anti-LDHB-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von LDHB in WB, IP, IF, EIA, RIA, DB und ID. Geeignet für Rind (Kuh).

Kurzübersicht für LDHB Antikörper (Biotin) (ABIN568275)

Target: LDHB (Lactate Dehydrogenase B (LDHB))

Reaktivität: Rind (Kuh)

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser LDHB Antikörper ist konjugiert mit Biotin

Applikation: Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF), Enzyme Immunoassay (EIA), Radioimmunoassay (RIA), Dot Blot (DB), Immunodiffusion (ID)

Produktdetails anti-LDHB Antikörper (Biotin)

Spezifität: L-Lactic Dehydrogenase from Bovine heart. The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: Immunoelectrophoresis, Cross-Immunoelectrophoresis, Single Radial Immunodiffusion (Ouchterlony), block titration, ELISA, Immunoblotting and enzyme inhibition. Cross-reactivities against enzymes of other sources may occur but have not been determined.

Produktmerkmale: Molar Ratio: Biotin/IgG ~5.6

Aufreinigung: Ammonium Sulphate Precipitation and Ion Exchange Chromatography

Immunogen: L-Lactic Dehydrogenase is isolated and purified from Bovine heart. Freund’s complete adjuvant is used in the first step of the immunization procedure.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 10 mg/mL

Buffer: PBS, pH 7.2 without preservatives and foreign proteins

Konservierungsmittel: Without preservative

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody lyophilized at 2-8 °C and reconstituted at 2-8 °C for one week or (in aliquots) at -20 °C for longer. If a slight precipitation occurs upon storage, this should be removed by centrifugation.

Details zu LDHB

Target: LDHB (Lactate Dehydrogenase B (LDHB))

Andere Bezeichnung: LDHB

Hintergrund: Lactate dehydrogenase (LDH) is present in a wide variety of organisms, including plants and animals. It is an oxidoreductase which catalyses the interconversion of pyruvate and lactate with concomitant interconversion of NADH and NAD+. As it can also catalyze the oxidation of hydroxybutyrate, it is occasionally called Hydroxybutyrate Dehydrogenase (HBD). There are 5 different isoenzymes of LDH, LDH1 to LDH5, each composed of 4 subunits which may be of 2 different types - M and H subunits. These subunits are encoded by two different genes: The M subunit is encoded by gene LDHA whilst the H subunit is encoded by LDHB. Usually LDH2 is the predominant form in the serum. An LDH1 level higher than the LDH2 level suggests myocardial infarction (damage to heart tissues releases heart LDH, which is rich in LDH1, into the bloodstream).Synonyms: EC=1.1.1.27, L-lactate dehydrogenase B chain, LDH heart subunit, LDH-B, LDH-H, LDHB, Renal carcinoma antigen NY-REN-46

Gen-ID: 281275

NCBI Accession: NP_776525

UniProt: Q5E9B1

Pathways: Warburg Effekt