LDHB Antikörper (C-Term)

Produktnummer ABIN6744435

Produktdetails anti-LDHB Antikörper

Target: LDHB (Lactate Dehydrogenase B (LDHB))

Bindungsspezifität: C-Term

Reaktivität: Human, Hund, Kaninchen, Affe, Huhn

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser LDHB Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Marke: IHC-plus™

Spezifität: Human LDHB

Homologie: Percent identity by BLAST analysis: Human, Dog, Rabbit, Chicken (100%) Mouse, Rat, Bovine, Horse, Pig (92%) Zebrafish (90%).

Aufreinigung: Affinity purified

Immunogen: Synthetic peptide from C-Terminus of human LDHB (P07195, NP_002291). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Ferret, Dolphin, Elephant, Panda, Dog, Rabbit, Opossum, Turkey, Chicken (100%), Galago, Mouse, Rat, Hamster, Bovine, Horse, Pig, Guinea pig (92%), Catfish (91%), Zebrafish (90%), Zebra finch (85%), Salmon (84%).

Type of Immunogen: Synthetic peptide

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-P (5 μg/mL), WB (1 μg/mL)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. ELISA titer using peptide based assay: 1:62500. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Distilled water

Konzentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Details zu LDHB

Target: LDHB (Lactate Dehydrogenase B (LDHB))

Andere Bezeichnung: LDHB / LDH-B (LDHB Produkte)

Synonyme: LDH-B antikoerper, LDH-H antikoerper, LDHBD antikoerper, TRG-5 antikoerper, AI790582 antikoerper, H-Ldh antikoerper, Ldh-2 antikoerper, Ldh2 antikoerper, ldh-h antikoerper, ldh2 antikoerper, ldhb antikoerper, ldhba antikoerper, trg-5 antikoerper, LDHB antikoerper, LDH-B-A antikoerper, wu:fa16d07 antikoerper, wu:fa20c10 antikoerper, wu:fa96a08 antikoerper, LDH-B-B antikoerper, zgc:92882 antikoerper, lactate dehydrogenase B antikoerper, lactate dehydrogenase B S homeolog antikoerper, lactate dehydrogenase Ba antikoerper, lactate dehydrogenase Bb antikoerper, LDHB antikoerper, Ldhb antikoerper, ldhb.S antikoerper, ldhb antikoerper, ldhba antikoerper, ldhbb antikoerper

Hintergrund: Name/Gene ID: LDHB

Synonyms: LDHB, LDH heart subunit, Lactate dehydrogenase H chain, Lactate dehydrogenase B, LDH-B, TRG-5, LDH-H, LDHBD

Gen-ID: 3945

NCBI Accession: NP_002291

UniProt: P07195

Pathways: Warburg Effekt