K-RAS Antikörper (AA 1-186)

Produktnummer ABIN5541964

Dieses Anti-K-RAS-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von K-RAS in WB, IF, FACS, ICC und EIA. Geeignet für Human.

Kurzübersicht für K-RAS Antikörper (AA 1-186) (ABIN5541964)

Target: K-RAS (KRAS) (GTPase Kras (KRAS))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser K-RAS Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC), Enzyme Immunoassay (EIA)

Klon: AT2F8

Produktdetails anti-K-RAS Antikörper

Bindungsspezifität: AA 1-186

Aufreinigung: Protein-A affinity chromatography

Immunogen: Recombinant human KRAS (1-186aa) purified from E. coli.

Anwendungsinformationen

Applikationshinweise: The antibody has been tested by ELISA, Western blot analysis, ICC/IF and Flow cytometry to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Liquid. In Phosphate-Buffered Saline ( pH 7.4) with 0.02 % Sodium Azide, 10 % Glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Haltbarkeit: 12 months

Details zu K-RAS

Target: K-RAS (KRAS) (GTPase Kras (KRAS))

Andere Bezeichnung: kras

Substanzklasse: Viral Protein

Hintergrund: KRAS acts as a molecular on/off switch. Once it is turned on, it recruits and activates proteins necessary for the propagation of growth factor and other receptors' sigl such as c-Raf and PI 3-kise. KRAS binds to GTP in the active state and possesses an intrinsic enzymatic activity which cleaves the termil phosphate of the nucleotide converting it to GDP. Upon conversion of GTP to GDP, KRAS is turned off. The rate of conversion is usually slow but can be sped up dramatically by an accessory protein of the GTPase-activating protein (GAP) class, for example RasGAP. In turn KRAS can bind to proteins of the Guanine Nucleotide Exchange Factor (GEF) class, for example SOS1, which forces the release of bound nucleotide (GDP). Subsequently, KRAS binds GTP present in the cytosol and the GEF is released from ras-GTP.

UniProt: P01116