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K-RAS Antikörper (AA 25-130)

Name: K-RAS Antikörper (AA 25-130)
SKU: ABIN736671
Price: 384.62 EUR
Availability: InStock

KRAS Reaktivität: Human, Ratte, Maus WB, ELISA, FACS, IF (cc), IF (p), IHC (p), IHC (fro) Wirt: Kaninchen Polyclonal unconjugated

Formalin-fixed and paraffin embedded: human endometrium tissue labeled with Anti-KRAS Polyclonal Antibody (ABIN736671), Unconjugated at 1:200, followed by conjugation to the secondary antibody and DAB staining (K-RAS Antikörper (AA 25-130))

IHC

Paraformaldehyde-fixed, paraffin embedded Mouse Kidney tissue, Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30min, Antibody incubation with KRAS Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining (K-RAS Antikörper (AA 25-130))

IHC (p)

Paraformaldehyde-fixed, paraffin embedded mouse kidney, Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30min, Antibody incubation with KRAS Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C, followed by a conjugated secondary (Goat Anti-rabbit IgG/Bio) for 20minutes at 37°C, followed by a conjugated streptavidin at 1:500 for 40 minutes and DAPI staining of the nuclei. (K-RAS Antikörper (AA 25-130))

IF (p)

3 Abbildungen

Produktnummer ABIN736671

Datenblatt (PDF)

Target Alle K-RAS (KRAS) Antikörper anzeigen

K-RAS (KRAS) (GTPase Kras (KRAS))
Bindungsspezifität
15

11

10

8

6

2

2

2

2

1

1

1

1

1

1
AA 25-130
Reaktivität
55

38

31

4

4

3

3

3

3

3

3

2

2

2

2

1

1

1
Human, Ratte, Maus
Wirt
57

11
Kaninchen
Passende Sekundärantikörper
Klonalität
56

12
Polyklonal
Konjugat
30

7

4

4

4

3

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
Dieser K-RAS Antikörper ist unkonjugiert
Applikation
62

29

24

16

12

12

9

6

5

5

2

1
Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Kreuzreaktivität

Human, Maus, Ratte

Aufreinigung

Purified by Protein A.

Immunogen

KLH conjugated synthetic peptide derived from human K-ras

Isotyp

IgG

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KRAS Reaktivität: Human WB, IHC Wirt: Kaninchen Polyclonal unconjugated

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KRAS Reaktivität: Human WB, IHC, IP, ICC Wirt: Kaninchen Polyclonal unconjugated

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KRAS Reaktivität: Human WB, ELISA Wirt: Maus Monoclonal 1E4-1B10 unconjugated

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KRAS Reaktivität: Human, Ratte, Maus WB, ELISA, IHC Wirt: Kaninchen Polyclonal unconjugated

Applikationshinweise

WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Beschränkungen

Nur für Forschungszwecke einsetzbar
Format

Liquid

Konzentration

1 μg/μL

Buffer

0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Konservierungsmittel

ProClin

Vorsichtsmaßnahmen

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Lagerung

4 °C,-20 °C

Informationen zur Lagerung

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Haltbarkeit

12 months
Target

K-RAS (KRAS) (GTPase Kras (KRAS))

Andere Bezeichnung

KRAS (KRAS Produkte)

Synonyme

wu:fa04e08 antikoerper, wu:fc14b12 antikoerper, wu:fc23g10 antikoerper, wu:fj89d12 antikoerper, zgc:85725 antikoerper, Kras2 antikoerper, c-Ki-ras antikoerper, p21 antikoerper, C-K-RAS antikoerper, CFC2 antikoerper, K-RAS2A antikoerper, K-RAS2B antikoerper, K-RAS4A antikoerper, K-RAS4B antikoerper, KI-RAS antikoerper, KRAS1 antikoerper, KRAS2 antikoerper, NS antikoerper, NS3 antikoerper, RASK2 antikoerper, AI929937 antikoerper, K-ras antikoerper, Ki-ras antikoerper, Kras-2 antikoerper, p21B antikoerper, ras antikoerper, k-ras antikoerper, kras antikoerper, kras-a antikoerper, kras-b antikoerper, rask2 antikoerper, K-RAS antikoerper, p21ras antikoerper, KRAS proto-oncogene, GTPase antikoerper, v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog antikoerper, Kirsten rat sarcoma viral oncogene homolog antikoerper, semicolonial-7 antikoerper, Ras family protein antikoerper, GTPase KRas antikoerper, Kirsten rat sarcoma viral oncogene homolog S homeolog antikoerper, KRAS antikoerper, kras antikoerper, Kras antikoerper, smco-7 antikoerper, PGTG_03066 antikoerper, Tsp_02666 antikoerper, Tsp_01642 antikoerper, rask antikoerper, kras.S antikoerper

Substanzklasse

Viral Protein

Hintergrund

Synonyms: NS, NS3, CFC2, KRAS1, KRAS2, RASK2, KI-RAS, C-K-RAS, K-RAS2A, K-RAS2B, K-RAS4A, K-RAS4B, GTPase KRas, K-Ras 2, c-Ki-ras, KRAS
Background: Ras proteins bind GDP/GTP and possess intrinsic GTPase activity. Plays an important role in the regulation of cell proliferation (PubMed:23698361, PubMed:22711838).

Gen-ID

3845

UniProt

P01116