COL6 Antikörper

Produktnummer ABIN5541083

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch COL6 in IHC (p), IHC (fro), IF, EIA, ICC und RIA. Er zeigt eine Reaktivität gegenüber Human, Maus, Ratte, Rind (Kuh), Huhn und Schwein.

Kurzübersicht für COL6 Antikörper (ABIN5541083)

Target: COL6 (Collagen, Type VI (COL6))

Reaktivität: Human, Maus, Ratte, Rind (Kuh), Huhn, Schwein

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser COL6 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Enzyme Immunoassay (EIA), Immunocytochemistry (ICC), Radioimmunoassay (RIA)

Produktdetails anti-COL6 Antikörper

Spezifität: Human Collagen type VI: 100 % binding in RIA at 1/10,000 dilution.

Aufreinigung: Affinity Chromatography.

Immunogen: Purified collagen type VI from human placenta.

Anwendungsinformationen

Applikationshinweise: ELISA: 1/200 (OD ≥ 500). RIA. Immunofluorescence: 1/80. Immunohistology on Frozen and Paraffin Embedded Sections. For paraffin: 1/400-1/1000, 60 min at RT or 2-8 °C over night for human collagen type VI, lower dilution is recommended for other species). Positive Control: Human placenta. Pretreatment : After de-waxing the tissue slices are treated with 0.2 % hyaluronidase (app. 300 U/mg) in TBS 15 min at 37 °C. There after non-specific binding is blocked by blocking serum or 3 % BSA in TBS. For peroxidase systems blocking with 1 % peroxide solution in TBS for 30 min at RT is recommended.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Buffer: PBS containing no BSA or preservative.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Haltbarkeit: 12 months

Details zu COL6

Target: COL6 (Collagen, Type VI (COL6))

Andere Bezeichnung: collagen type vi

Hintergrund: Collagens consist in a family of highly specialized glycoproteins of which at least 16 genetically distinct types are known to date. The basal unit of a collagen molecule cosists in a tripel-helical structure formed by 3 alpha-chains. Predominant amino acids are glycine, proline and hydroxproline. Regularly also lysines and hydroxylysines occur, which are responsible for cross-linkage and glycosylation of the protein chains. Different composition of alpha-chains and different glycosylation contribute to the high variability of collagens in different tissues and organs. Collagen type VI is found as 5-10 nm diameter microfibrils (periodicity 100 nm) e.g. in placenta, skin, cartilage and cornea.

UniProt: P12109