COL6 Antikörper

Produktnummer ABIN5680257

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch COL6 in WB, ELISA, IF, IHC (p) und RIA. Es zeigt Reaktivität gegenüber Proben von Human.

Kurzübersicht für COL6 Antikörper (ABIN5680257)

Target: COL6 (Collagen, Type VI (COL6))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser COL6 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Radioimmunoassay (RIA)

Produktdetails anti-COL6 Antikörper

Spezifität: Human collagen type VI 100 % binding in RIA at 1:10.000 dilution, cross-reactivity with human collagen type I, II, III, IV and V <0.1 %, human elastin and human laminin <0.1 %, human fibronectin < 1 % Human, cross-reactive with bovine

Kreuzreaktivität: Huhn, Rind (Kuh), Maus, Schwein, Ratte (Rattus)

Kreuzreaktivität (Details): Human (cross-reactive with bovine, porcine, chicken, murine and rat tissue)

Produktmerkmale: Collagen type VI is found as 5-10 nm diameter microfibrils (periodicity 100 nm) e.g. in placenta, skin, cartilage and cornea. Collagens consist of a family of highly specialized glycoproteins of which at least 16 genetically distinct types are known to date. The basal unit of a collagen molecule consists of a triple-helical structure formed by 3 alpha-chains. Predominant amino acids are glycine, proline and hydroxproline. Regularly also lysines and hydroxylysines occur, which are responsible for cross-linkage and glycosylation of the protein chains. Different composition of alpha-chains and different glycosylation contribute to the high variability of collagens in different tissues and organs. Human collagen type VI 100 % , human collagen type I, II, III, IV and V <0.1 %, human elastin and human laminin <0.1 %, human fibronectin < 1 % (RIA at 1:10.000 dilution).

Aufreinigung: Affinity purified

Immunogen: Purified collagen type VI from human placenta

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: After de-waxing the tissue slices they are treated with 0.2 % hyaluronidase (app. 300 U/mg ) in TBS 15 min at 37 °C. Thereafter non-specific binding is blocked by blocking serum or 3 % BSA in TBS. For peroxidase systems blocking with 1 % peroxide solution in TBS for 30 min at RT is recommended.,Human placenta,IHC(P), IFA, ELISA, RIA, IB/WB,IHC(P) 60 min at RT or 2-8 °C over night,(purified, lyophilized) 1:80 IFA, 1:500 - 1:1000 IHC(P)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Konzentration: 1 mg/mL

Buffer: phosphate buffered solution, no BSA and preservative added!

Konservierungsmittel: Without preservative

Lagerung: -20 °C

Details zu COL6

Target: COL6 (Collagen, Type VI (COL6))

Andere Bezeichnung: Collagen Type VI