PKM2 Antikörper (AA 1-531)

Produktnummer ABIN5540840

Dieses Maus Monoklonal-Antikörper erkennt spezifisch PKM2 in WB und EIA. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für PKM2 Antikörper (AA 1-531) (ABIN5540840)

Target: PKM2 (Pyruvate Kinase M2 (PKM2))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser PKM2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Enzyme Immunoassay (EIA)

Klon: AT1B10

Produktdetails anti-PKM2 Antikörper

Bindungsspezifität: AA 1-531

Aufreinigung: Protein-A affinity chromatography

Immunogen: Recombinant human PKM2 (1-531aa) purified from E.coli.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: The antibody has been tested by ELISA, Western blot analysis and Immunofluorescence analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended starting dilution for Western blot analysis is 1:1000 and Immunofluorescence is 1:250.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: PBS, pH 7.4 containing 0.02 % Sodium Azide and 10 % Glycerol

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store undiluted at 2-8°C for up to two weeks or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

Haltbarkeit: 12 months

Details zu PKM2

Target: PKM2 (Pyruvate Kinase M2 (PKM2))

Andere Bezeichnung: pkm2

Hintergrund: PKM2 (pyruvate kinase M2), its association with phospho-tyrosine (pTyr) motifs, enables cells to use predominantly aerobic glycolysis instead of oxidative phosphorylation. Also PKM2 suggest that this change benefits cancer cells by enabling them to redirect glycolytic metabolites away from oxidation and energy production and, instead, toward anabolic processes and biosynthesis. PKM2-mediated generation of the aerobic glycolytic phenotype, although wasteful in terms of glucose consumption and waste generation, could provide a variety of advantages for cancerous cells. In addition nuclear translocation of the tumor marker pyruvate kinase M2 induces programmed cell death.

UniProt: P14618

Pathways: Warburg Effekt