Metabotropic Glutamate Receptor 5 Antikörper
Kurzübersicht für Metabotropic Glutamate Receptor 5 Antikörper (ABIN5540473)
Target
Alle Metabotropic Glutamate Receptor 5 (GRM5) Antikörper anzeigenReaktivität
Wirt
Klonalität
Konjugat
Applikation
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Aufreinigung
- Affinity Chromatography
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Immunogen
- Chickens were immunized with three synthetic peptide / keyhole limpet hemocyanin (KLH) conjugates. Production: These synthetic peptides corresponded to different regions of the metabotropic glutamate receptor type 5 gene product, but are shared between the Human (NP_001137303.1, NCBI) and Mouse (Q3UVX5.2, NCBI) sequences. After repeated injections, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column, and the concentrations of the eluates adjusted to 0.3 mg/ml. Finally, equal volumes of these affinity-purified anti-peptide antibodies were mixed, and the preparation was filter-sterilized.
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Isotyp
- Ig Fraction
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Applikationshinweise
- Quality Control: This anti-peptide antibody mixture was validated by Immunohistochemistry (at 1/2000 dilution) using Fluorescein Conjugated Affinity-purified Goat anti-Chicken IgY antibody (Cat.-No AP31795FCN, 1/5000 dilution) as a secondary antibody. Recommended Dilutions: 1/1000-1/2000 for Immunohistochemistry and Immunocytochemistry using 2 % paraformaldehyde-fixed tissues or cells.
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Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Format
- Liquid
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Konservierungsmittel
- Sodium azide
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Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Lagerung
- 4 °C
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Informationen zur Lagerung
- Store undiluted at 2-8°C. This product is photosensitive and should be protected from light. Shelf life: one year from despatch.
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Haltbarkeit
- 12 months
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- Metabotropic Glutamate Receptor 5 (GRM5) (Glutamate Receptor, Metabotropic 5 (GRM5))
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Andere Bezeichnung
- mglur5,grm5
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UniProt
- P41594
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Pathways
- Regulation of G-Protein Coupled Receptor Protein Signaling, Regulation of long-term Neuronal Synaptic Plasticity
Target
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