C5AR1 Antikörper (C-Term)

Produktnummer ABIN5539670

Dieses Ziege Polyklonal-Antikörper erkennt spezifisch C5AR1 in WB, FACS, ELISA, IHC und IF. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für C5AR1 Antikörper (C-Term) (ABIN5539670)

Target: C5AR1 (Complement Component 5a Receptor 1 (C5AR1))

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser C5AR1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Flow Cytometry (FACS), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Güteklasse: Verified

Produktdetails anti-C5AR1 Antikörper

Bindungsspezifität: C-Term

Verwendungszweck: C5AR1/CD88

Sequenz: RESKSFTRST VDT

Kreuzreaktivität: Human

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry: Paraffin embedded Human Spleen. Recommended concentration: 6-8 μg/mL.

Western Blot: Approx 38 kDa band observed in lysates of cell line HepG2 (calculated MW of 39.3 kDa according to NP_001727.1). Recommended concentration: 0.01-0.03 μg/mL. Primary incubation 1 hour at room temperature.

Peptide ELISA: antibody detection limit dilution 1:64000.

Kommentare:

Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HepG2 cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of HepG2 cells. Recommended concentration: 10ug/m

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Details zu C5AR1

Target: C5AR1 (Complement Component 5a Receptor 1 (C5AR1))

Andere Bezeichnung: C5AR1

Hintergrund: C5AR1, C5A, C5AR, C5A-R, C5R1, CD88, C5a anaphylatoxin chemotactic receptor, C5a anaphylatoxin receptor, Complement component 5 receptor 1, Complement component 5a receptor 1, Anaphylatoxin receptor

Gen-ID: 728, 113959

NCBI Accession: NP_001727

Pathways: Komplementsystem