EGF Antikörper (Internal Region)

Produktnummer ABIN5539508

Dieses Ziege Polyklonal-Antikörper erkennt spezifisch EGF in WB, IHC und ELISA. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für EGF Antikörper (Internal Region) (ABIN5539508)

Target: EGF (Epidermal Growth Factor (EGF))

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser EGF Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Güteklasse: Verified

Produktdetails anti-EGF Antikörper

Bindungsspezifität: Internal Region

Verwendungszweck: Pro-EGF

Sequenz: SRQERVCNIE KNVS

Spezifität: This antibody is expected to recognize all reported isoforms (NP_001954.2, NP_001171601.1, NP_001171602.1), but not the mature form. The immunizing peptide represents part of the extracellular domain.

Kreuzreaktivität: Hund, Human

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry: Paraffin embedded Human Skin. Recommended concentration: 5 μg/mL.

Western Blot: Approx 160 kDa double band observed in Human Prostate lysates (calculated MW of 134 kDa according to NP_001954.2). The two bands represent glycosylated forms of the mature protein. Recommended concentration: 1-3 μg/mL. Primary incubation was 1

Peptide ELISA: antibody detection limit dilution 1:128000.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Details zu EGF

Target: EGF (Epidermal Growth Factor (EGF))

Andere Bezeichnung: EGF

Hintergrund: EGF, epidermal growth factor, HOMG4, URG, OTTHUMP00000162922, OTTHUMP00000219721, OTTHUMP00000219722, beta-urogastrone, pro-epidermal growth factor

Gen-ID: 1950

NCBI Accession: NP_001954, NP_001171601, NP_001171602

Pathways: NF-kappaB Signalweg, RTK Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation