Leptin Antikörper (AA 22-167)

Produktnummer ABIN5518931

Dieses Anti-Leptin-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von Leptin in WB und ELISA. Geeignet für Ratte. Dieses Primary Antibody wurde in 1 Publikation zitiert.

Kurzübersicht für Leptin Antikörper (AA 22-167) (ABIN5518931)

Target: Leptin (LEP)

Reaktivität: Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Leptin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Produktdetails anti-Leptin Antikörper

Bindungsspezifität: AA 22-167

Verwendungszweck: Anti-Leptin Antibody Picoband®

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-Leptin Antibody Picoband® (ABIN5518931). Tested in ELISA, WB applications. This antibody reacts with Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: E. coli-derived rat Leptin recombinant protein (Position: V22-C167). Rat Leptin shares 83.6% and 95.9% amino acid (aa) sequence identity with human and mouse Leptin, respectively.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: ELISA, 0.1-0.5 μg/mL, Western blot, 0.1-0.5 μg/mL, Rat,
1. Halaas JL, Gajiwala KS, Maffei M, Cohen SL, Chait BT, Rabinowitz D, Lallone RL, Burley SK, Friedman JM (July 1995). "Weight-reducing effects of the plasma protein encoded by the obese gene". Science 269 (5223): 543-6. 2. Maffei M, Halaas J, Ravussin E, Pratley RE, Lee GH, Zhang Y, Fei H, Kim S, Lallone R, Ranganathan S (November 1995). "Leptin levels in human and rodent: measurement of plasma leptin and ob RNA in obese and weight-reduced subjects". Nat. Med. 1 (11): 1155-61. 3. Pan H, Guo J, Su Z (May 2014). "Advances in understanding the interrelations between leptin resistance and obesity".Physiology & Behavior 130: 157-169.

Kommentare:

We recommend Enhanced Chemiluminescent Kit with anti-Rabbit IgG (ABIN921124) for Western blot.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Referenzen für anti-Leptin Antikörper (ABIN5518931)

Li, Zhao, Qi, Wang, Zhang, Li, Qin: "lncRNA Ftx promotes aerobic glycolysis and tumor progression through the PPARγ pathway in hepatocellular carcinoma." in: International journal of oncology, Vol. 53, Issue 2, pp. 551-566, (2018) (PubMed).

Details zu Leptin

Target: Leptin (LEP)

Andere Bezeichnung: Lep

Hintergrund:

Synonyms: Leptin,Obesity factor,Lep,Ob,

Tissue Specificity: Expressed in the parathyroid.

Background: Leptin have been found to be obese and diabetic and to have reduced activity, metabolism and body temperature. cDNA clones encoding Leptin have been isolated from human, simian, mouse, and rat cells. The expression of Leptin mRNA has been shown to be restricted to adipose tissue. Although regulation of fat stores is deemed to be the primary function of leptin, it also plays a role in other physiological processes, as evidenced by its multiple sites of synthesis other than fat cells, and the multiple cell types beside hypothalamic cells that have leptin receptors. Many of these additional functions are yet to be defined.

Molekulargewicht: 16 kDa

Gen-ID: 25608

UniProt: P50596

Pathways: JAK-STAT Signalweg, AMPK Signaling, Hormone Transport, Peptide Hormone Metabolism, Hormone Activity, Negative Regulation of Hormone Secretion, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Monocarboxylic Acid Catabolic Process