Cytokeratin 18 Antikörper (AA 204-430)

Produktnummer ABIN5518769

Produktdetails anti-Cytokeratin 18 Antikörper

Target: Cytokeratin 18 (KRT18) (Keratin 18 (KRT18))

Bindungsspezifität: AA 204-430

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Cytokeratin 18 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Verwendungszweck: Rabbit IgG polyclonal antibody for Keratin, type I cytoskeletal 18(KRT18) detection. Tested with WB, IHC-P in Human,Mouse,Rat.

Kreuzreaktivität (Details): No cross reactivity with other proteins.

Produktmerkmale: Rabbit IgG polyclonal antibody for Keratin, type I cytoskeletal 18(KRT18) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: keratin 18
Protein Name: Keratin, type I cytoskeletal 18

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human Cytokeratin 18 recombinant protein (Position: E204-H430). Human Cytokeratin 18 shares 87.7% and 85.9% amino acid (aa) sequence identity with mouse and rat Cytokeratin 18, respectively.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Mouse, Rat
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Referenzen für anti-Cytokeratin 18 Antikörper (ABIN5518769)

Li, Li, Sun, Wu, Wang: "Comparison of the effect of recombinant bovine wild and mutant lipopolysaccharide-binding protein in lipopolysaccharide-challenged bovine mammary epithelial cells." in: Cell stress & chaperones, Vol. 21, Issue 3, pp. 439-52, (2017) (PubMed).

Zhang, Liu, Liu, Zhou, Song, Cao, An: "miR-182 aids in receptive endometrium development in dairy goats by down-regulating PTN expression." in: PLoS ONE, Vol. 12, Issue 7, pp. e0179783, (2017) (PubMed).

Wu, Guo, Hui, Ye, Xiang, Wan, Zou, Ma, Sun, Yang, Guo: "Stanniocalcin-1 protects bovine intestinal epithelial cells from oxidative stress-induced damage." in: Journal of veterinary science, Vol. 15, Issue 4, pp. 475-83, (2015) (PubMed).

Xu, Jin, Min, Li, Deng, Wu, Lin, Chen, Zhang, Li, Wang, Zhu, Wang, Chu, Shen, Li, Mao: "Chloride channel-3 promotes tumor metastasis by regulating membrane ruffling and is associated with poor survival." in: Oncotarget, Vol. 6, Issue 4, pp. 2434-50, (2015) (PubMed).

Yao, Guo, Wang, Xu, Zhang, Li, Lai: "The Paracrine Effect of Transplanted Human Amniotic Epithelial Cells on Ovarian Function Improvement in a Mouse Model of Chemotherapy-Induced Primary Ovarian Insufficiency." in: Stem cells international, Vol. 2016, pp. 4148923, (2015) (PubMed).

Hu, Zhou, Shi, Zhu, Wang, Zhang, Hu, Qian, Xu: "Mesenchymal stem-like cells isolated from human esophageal carcinoma and adjacent non-cancerous tissues." in: Oncology letters, Vol. 5, Issue 1, pp. 179-184, (2012) (PubMed).

Details zu Cytokeratin 18

Target: Cytokeratin 18 (KRT18) (Keratin 18 (KRT18))

Andere Bezeichnung: KRT18 (KRT18 Produkte)

Synonyme: CK18 antikoerper, K18 antikoerper, Krt1-18 antikoerper, CYK18 antikoerper, DreK18 antikoerper, cb83 antikoerper, dapk1 antikoerper, sb:cb83 antikoerper, wu:fa13f02 antikoerper, wu:fb36b04 antikoerper, krt18 antikoerper, MGC64569 antikoerper, MGC75922 antikoerper, KRT18 antikoerper, CK-18-A antikoerper, K18-A antikoerper, NC-11 antikoerper, cyk18 antikoerper, k18 antikoerper, krt18-a antikoerper, krt18-b antikoerper, krt18a antikoerper, xkendob antikoerper, keratin 18 antikoerper, keratin 18, type I L homeolog antikoerper, keratin 18, type I antikoerper, keratin, type I cytoskeletal 18 antikoerper, K18, simple type I keratin antikoerper, Keratin, type I cytoskeletal 18 antikoerper, keratin 18, type I S homeolog antikoerper, Krt18 antikoerper, KRT18 antikoerper, krt18 antikoerper, krt18.L antikoerper, LOC700569 antikoerper, LOC100136778 antikoerper, k1c18 antikoerper, LOC100008885 antikoerper, krt18.S antikoerper

Hintergrund: Keratin 18, mapped to 12q13.13, is a type I cytokeratin. It is, together with its filament partner keratin 8, perhaps the most commonly found products of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene.

Synonyms: CK18 | CK-18 | CK 18 | CYK 18 | CYK18 | Cytokeratin 18 | Cytokeratin endo B | Cytokeratin-18 | K 18 | K18 | KA18 | Keratin 18 | Keratin D | Keratin-18 | Krt18 | P05783

Gen-ID: 3875

UniProt: P05783

Pathways: Apoptose, Caspase Kaskade in der Apoptose