EPAS1 Antikörper (Middle Region)

Produktnummer ABIN5518650

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch EPAS1 in WB. Er zeigt eine Reaktivität gegenüber Human, Ratte und Maus und wurde in 1 Publikation erwähnt.

Kurzübersicht für EPAS1 Antikörper (Middle Region) (ABIN5518650)

Target: EPAS1 (Endothelial PAS Domain Protein 1 (EPAS1))

Reaktivität: Human, Ratte, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser EPAS1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-EPAS1 Antikörper

Bindungsspezifität: AA 282-301, Middle Region

Verwendungszweck: Anti-HIF-2-alpha/EPAS1 Antibody Picoband®

Sequenz: HALDSENMTK SHQNLCTKGQ

Kreuzreaktivität (Details): No cross-reactivity with other proteins

Produktmerkmale: Anti-HIF-2-alpha/EPAS1 Antibody (ABIN5518650). Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human HIF-2-alpha, identical to the related mouse and rat sequence.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
1. Tian, H., McKnight, S. L., Russell, D. W. : Endothelial PAS domain protein 1 (EPAS1), a transcription factor selectively expressed in endothelial cells. Genes Dev. 11: 72-82, 1997. 2. Sood, R., Zehnder, J. L., Druzin, M. L., Brown, P. O. : Gene expression patterns in human placenta. Proc. Nat. Acad. Sci. 103: 5478-5483, 2006. 3. Henderson, J., Withford-Cave, J. M., Duffy, D. L., Cole, S. J., Sawyer, N. A., Gulbin, J. P., Hahn, A., Trent, R. J., Yu, B. : The EPAS1 gene influences the aerobic-anaerobic contribution in elite endurance athletes. Hum. Genet. 118: 416-423, 2005.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Konservierungsmittel: Sodium azide, Thimerosal (Merthiolate)

Vorsichtsmaßnahmen: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Referenzen für anti-EPAS1 Antikörper (ABIN5518650)

Lu, Shi, Ma, Wang, Liu, Chuai, Song, Münsterberg, Cao, Yang: "Proper autophagy is indispensable for angiogenesis during chick embryo development." in: Cell cycle (Georgetown, Tex.), Vol. 15, Issue 13, pp. 1742-54, (2017) (PubMed).

Details zu EPAS1

Target: EPAS1 (Endothelial PAS Domain Protein 1 (EPAS1))

Andere Bezeichnung: EPAS1

Hintergrund:

Synonyms: Endothelial PAS domain-containing protein 1,EPAS-1,Hypoxia-inducible factor 2-alpha,HIF-2-alpha,HIF2-alpha,Epas1,Hif2a,

Background: HIF-2 alpha is also designated EPAS1 whose gene is mapped to 2p21-p16. The predicted mouse protein is 88 % identical to human EPAS1. The human EPAS1 gene contains 15 exons and spans at least 120 kb. The positions of the introns within the genomic region encoding the N-terminal bHLH-PAS domains of EPAS1 and AHR are similar, suggesting that the 5-prime ends of the 2 genes may have arisen from a gene duplication event1. Moreover, the predicted protein shares 48 % sequence identity with HIF1-alpha, a bHLH-PAS transcription factor that induces EPO gene expression in cultured cells in response to hypoxia. Like HIF1A, EPAS1 binds to and activates transcription from the HIF1A response element derived from the 3-prime flanking region of the EPO gene. EPAS1 is predominantly expressed in highly vascularized tissues of adult humans and in endothelial cells of the mouse adult and embryo. Furthermore, EPAS1 may represent an important regulator of vascularization, perhaps involving the regulation of endothelial cell gene expression in response to hypoxia2. HIF2A is expressed at relatively higher levels in villus sections of placenta and in lung samples compared with other tissues examined3. In addition, The variation in EPAS1 influences the relative contribution of aerobic and anaerobic metabolism and hence the maximum sustainable metabolic power for a given event duration4.

Sequence Similarities: Contains 1 bHLH (basic helix-loop-helix) domain.

Molekulargewicht: 120 kDa

Pathways: Signaling Events mediated by VEGFR1 and VEGFR2, Warburg Effekt