SKA3 Antikörper (C-Term)

Produktnummer ABIN500732

Der Kaninchen Polyklonal Anti-SKA3-Antikörper wurde für WB und EIA validiert. Er ist geeignet, SKA3 in Proben von Human, Ratte und Maus zu detektieren.

Kurzübersicht für SKA3 Antikörper (C-Term) (ABIN500732)

Target: SKA3 (Spindle and Kinetochore Associated Complex Subunit 3 (SKA3))

Reaktivität: Human, Ratte, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser SKA3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Enzyme Immunoassay (EIA)

Produktdetails anti-SKA3 Antikörper

Bindungsspezifität: C-Term

Spezifität: This antibody detects SKA3.

Kreuzreaktivität (Details): Species reactivity (tested):Human, mouse, rat

Aufreinigung: Peptide affinity chromatography

Immunogen: SKA3 antibody was raised against a 13 amino acid peptide from near the carboxy terminus of human SKA3.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: ELISA. Western blot: 0.5 - 1 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: PBS containing 0.02 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.

Details zu SKA3

Target: SKA3 (Spindle and Kinetochore Associated Complex Subunit 3 (SKA3))

Andere Bezeichnung: SKA3

Hintergrund: Upon entry into mitosis, the cell's microtubule (MT) network forms the mitotic spindle, allowing the segregation of paired chromosomes. Proteinaceous structures on centromeric chromatin termed kinetochores (KT) are essential for the proper attachment of the chromosomes to the spindle MTs. A recently discovered spindle and kinetochore complex, comprised of proteins SKA1, SKA2, and SKA3, has been found to be required for stable KT-MT interactions and timely anaphase onset. Like with SKA1 or SKA2, depletion of SKA3 by siRNA delays anaphase transition, resulting in a prolonged a metaphase-like state. These SKA3-depleted cells accumulate high levels of the checkpoint protein Bub1 at kinetochores, suggesting the SKA complex plays a key role in spindle checkpoint silencing and the maintenance of chromosome cohesion in mitosis.Synonyms: C13orf3, RAMA1, Spindle and kinetochore-associated protein 3

Gen-ID: 221150

NCBI Accession: NP_659498

UniProt: Q8IX90