RNASEH2A Antikörper (Center)

Produktnummer ABIN500622

Produktdetails anti-RNASEH2A Antikörper

Target: RNASEH2A (Ribonuclease H2, Subunit A (RNASEH2A))

Bindungsspezifität: Center

Reaktivität: Human, Ratte, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser RNASEH2A Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF)

Spezifität: This antibody detects RNAse H2A.

Kreuzreaktivität (Details): Species reactivity (tested):Human, mouse, rat

Aufreinigung: Peptide affinity chromatography

Immunogen: RNAse H2A antibody was raised against a 17 amino acid peptide near the center of human RNAse H2A.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: ELISA. Western blot: 1 - 2 μg/mL. Immunocytochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: PBS containing 0.02 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store at 2 - 8 °C for up to 3 months, and at -20 °C thereafter.

Referenzen für anti-RNASEH2A Antikörper (ABIN500622)

Zimmermann, Murina, Reijns, Agathanggelou, Challis, Tarnauskaitė, Muir, Fluteau, Aregger, McEwan, Yuan, Clarke, Lambros, Paneesha, Moss, Chandrashekhar, Angers, Moffat, Brunton, Hart, de Bono et al.: "CRISPR screens identify genomic ribonucleotides as a source of PARP-trapping lesions. ..." in: Nature, Vol. 559, Issue 7713, pp. 285-289, (2019) (PubMed).

Bartsch, Damme, Regen, Becker, Garrett, Hölter, Knittler, Borowski, Waisman, Glatzel, Fuchs, Gailus-Durner, Hrabe de Angelis, Rabe: "RNase H2 Loss in Murine Astrocytes Results in Cellular Defects Reminiscent of Nucleic Acid-Mediated Autoinflammation." in: Frontiers in immunology, Vol. 9, pp. 587, (2018) (PubMed).

Cuadrado, Michailidou, van Bodegraven, Jansen, Sluijs, Geerts, Couraud, De Filippis, Vescovi, Kuijpers, Hol: "Phenotypic variation in Aicardi-Goutières syndrome explained by cell-specific IFN-stimulated gene response and cytokine release." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 194, Issue 8, pp. 3623-33, (2015) (PubMed).

Details zu RNASEH2A

Target: RNASEH2A (Ribonuclease H2, Subunit A (RNASEH2A))

Andere Bezeichnung: RNASEH2A (RNASEH2A Produkte)

Hintergrund: Ribonucleases (RNAses) H are enzymes that hydrolyze the RNA strands of RNA/DNA hybrids. The major role of these enzymes is to remove the RNA strand from the RNA/DNA hybrids that form during DNA replication and repair. RNAse H2 is made up of three subunits, all three are required for RNAse activity. Recent evidence has demonstrated that mutations in RNAse H2A or any of the other subunits result in Aicardi-Goutieres syndrome (AGS), a neurological disorder with similar symptoms to viral brain infections including high levels of IFN-alpha in the cerebral spinal fluid. Similar conditions are observed with mutations in TREX1, a single-stranded DNA exonuclease, suggesting that RNAse H2 and TREX1 may have similar roles, and that mutations in any of these genes lead to an accumulation of intracellular nucleic acids, triggering an inflammatory response through activation of the innate immune system.Synonyms: AGS4, Aicardi-Goutieres syndrome 4 protein, RNASEHI, RNAse H2A, RNHIA, RNase H2 subunit A, RNase HI large subunit, Ribonuclease H2 subunit A, Ribonuclease HI large subunit, Ribonuclease HI subunit A

Gen-ID: 10535

UniProt: O75792