MYD88 Antikörper (AA 233-248)

Produktnummer ABIN500300

Der Kaninchen Polyklonal anti-MYD88 Antikörper wird verwendet zum Nachweis von MYD88 in Proben von Human und Maus. Er wurde validiert für WB, IHC (p) und EIA.

Kurzübersicht für MYD88 Antikörper (AA 233-248) (ABIN500300)

Target: MYD88 (Myeloid Differentiation Primary Response Gene (88) (MYD88))

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser MYD88 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Produktdetails anti-MYD88 Antikörper

Bindungsspezifität: AA 233-248

Spezifität: This antibody detects MYD88 at center.

Kreuzreaktivität (Details): Species reactivity (tested):Human, mouse

Aufreinigung: Peptide affinity chromatography

Immunogen: MyD88 antibody was raised against a peptide corresponding to amino acids 233 to 248 of human MyD88, which differ from those of mouse by two amino acids.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: ELISA. Western blot: 1: 500 - 1: 1000 dilution, whole cell lysate from Jurkat cells can be used aspositive control and an approximately 35 kDa band can be detected. Immunohistochemistry on paraffin sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: PBS containing 0.02 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.

Details zu MYD88

Target: MYD88 (Myeloid Differentiation Primary Response Gene (88) (MYD88))

Andere Bezeichnung: MYD88

Hintergrund: The pro-inflammatory cytokine IL-1 induced cellular response requires IL-1 receptor complex including IL-1RI and IL-1RAcP. Recently, MyD88 was identified as an adapter molecule in the IL-1 signaling pathway (1). MyD88 associates with and recruits IRAK to the IL-1 receptor complex in response to IL-1 treatment and dominant negative form of MyD88 attenuates IL-1R-mediated NF-kappaB activation. MyD88 is also employed as a regulator molecule by IL-18 receptor and human Toll receptor (2,3), which are members in the Toll/IL-1R family of receptors. Targeted disruption of the MyD88 gene results in lose of cellular responses to IL-1 and IL-18, and MyD88-deficient mice lack responses to bacterial product LPS that employs Toll-like receptors 2 and 4 (TLR2 and TLR4) as the signaling receptors. MyD88 is a general adapter protein for the Toll/IL-1R family of receptors and plays an important role in the inflammatory response induced by cytokines IL-1 and IL-18 and endotoxin. MyD88 gene is expressed in many tissues.Synonyms: Myeloid differentiation primary response protein MyD88

Gen-ID: 4615

UniProt: Q99836

Pathways: NF-kappaB Signalweg, TLR Signalweg, Neurotrophin Signalübertragung, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Toll-Like Receptors Cascades