IL-18 Antikörper (Internal Region)

Produktnummer ABIN499167

Zitiert in 1 Publikation. Der Ziege Polyklonal anti-IL-18 Antikörper (ABIN499167) detektiert spezifisch IL-18 in WB. Dieser Antikörper reagiert spezifisch mit Proben aus Human, Ratte und Kaninchen.

Kurzübersicht für IL-18 Antikörper (Internal Region) (ABIN499167)

Target: IL-18 (IL18) (Interleukin 18 (IL18))

Reaktivität: Human, Ratte, Kaninchen

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser IL-18 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-IL-18 Antikörper

Bindungsspezifität: Internal Region

Sequenz: C-NPPDNIKDTK SDI

Spezifität: This antibody detects Interleukin-18.

Kreuzreaktivität (Details): Species reactivity (expected):Rabbit, ratSpecies reactivity (tested):Human

Aufreinigung: Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Immunogen: Peptide from the internal region of the protein sequence according to NP_001553.1.

Anwendungsinformationen

Applikationshinweise: Peptide ELISA: limit dilution 1: 32000. Western blot: 1-3 μg/mL. Approx 23 kDa band observed in Human Placenta lysates and inlysates of cell line HeLa (calculated MW of 22.3 kDa according to NP_001553.1).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store at 2 - 8 °C up to one week or (in aliquots) at -20 °C for longer.

Referenzen für anti-IL-18 Antikörper (ABIN499167)

Rather, Malik, Trikha, Bhat, Dhawan: "Aqueous Terminalia arjuna extract modulates expression of key atherosclerosis-related proteins in a hypercholesterolemic rabbit: A proteomic-based study." in: Proteomics. Clinical applications, Vol. 10, Issue 7, pp. 750-9, (2017) (PubMed).

Details zu IL-18

Target: IL-18 (IL18) (Interleukin 18 (IL18))

Andere Bezeichnung: Interleukin-18 / IL18

Hintergrund: IL18 (IFN-gamma-inducing factor, IGIF) is a novel pro-inflammatory cytokine that augments natural killer (NK) activity in spleen cells. This cytokine can induce the IFN-gamma production of T cells. The combination of this cytokine and IL12 has been shown to inhibit IL4 dependent IgE and IgG1 production, and enhance IgG2a production of B cells. IL18 binding protein (IL18BP) can specifically interact with this cytokine, and thus negatively regulate its biological activity. IL18 also has other biological properties such as stimulation of Fas ligand expression, and induction of CC and CXC chemokines. IL18 increases production of competent HIV. IL18 is related to the IL1 family. One of the IL18 receptor chains is the IL1 receptor-related protein, a member of the IL 1R family. Like IL1 , IL18 is first synthesized as a leaderless precursor requiring the IL1 converting enzyme (ICE) for cleavage into an active molecule. IL18 may play a role in tissue injury in inflammatory reactions.Synonyms: IGIF, IL-1 gamma, IL-18, IL1 gamma, IL1F4, Iboctadekin, Interferon-gamma-inducing factor, Interleukin-1 gamma

Gen-ID: 3606

NCBI Accession: NP_001553

UniProt: Q14116

Pathways: Cellular Response to Molecule of Bacterial Origin, Activated T Cell Proliferation, Cancer Immune Checkpoints, Inflammasome