PDGFRB Antikörper (pTyr751)

Produktnummer ABIN498798

Der Kaninchen Polyklonal anti-PDGFRB Antikörper (ABIN498798) detektiert spezifisch PDGFRB in WB und IHC (p). Dieser Antikörper reagiert spezifisch mit Proben aus Human, Maus und Ratte.

Kurzübersicht für PDGFRB Antikörper (pTyr751) (ABIN498798)

Target: PDGFRB (Platelet Derived Growth Factor Receptor beta (PDGFRB))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PDGFRB Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-PDGFRB Antikörper

Bindungsspezifität: pTyr751

Spezifität: This antibody detects endogenous levels of PDGFR beta protein only when phosphorylated at Tyr751.

Kreuzreaktivität (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Aufreinigung: Affinity chromatography

Reinheit: > 95 % by SDS-PAGE

Anwendungsinformationen

Applikationshinweise: Western Blot: 1/500 - 1/1000. Immunohistochemistry: 1/50 - 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu PDGFRB

Target: PDGFRB (Platelet Derived Growth Factor Receptor beta (PDGFRB))

Andere Bezeichnung: CD140b / PDGFRB

Hintergrund: Platelet-derived growth factor (PDGF) is a mitogen for mesenchyme- and gliaderived cells. PDGF consists of two chains, A and B, which dimerize to form functionally distinct isoforms PGDF-AA, PDGF-AB and PDGF-BB. These three isoforms bind with different affinities to two receptor types, PDGFR-alpha and -beta, which are endowed with protein tyrosine kinase domains. PDGFR-alpha can bind to both A and B subunits of PDGF, while PDGFR-beta can only bind the B subunit. Ligand binding promotes either homo- or heterodimerization of the PDGF receptors in a specific manner. PDGF-AA induces the dimerization of two alpha receptors, PDGF-AB induces dimerization of alpha-alpha and alpha-beta, and PDGF-BB induces the formation of three types of dimers, alpha-alpha, alpha-beta and beta-beta. Translocation of the PDGFR-beta gene with the TEL gene is linked with chronic myelomonocytic leukemia (CMML), a myelodysplastic syndrome, and demonstrates the oncogenic potential of the PDGF receptors.Synonyms: Beta-type platelet-derived growth factor receptor, CD140 antigen-like family member B, PDGF Receptor beta, PDGF-R-beta

Molekulargewicht: approx. 124, 190 kDa

Gen-ID: 5159

NCBI Accession: NP_002600

UniProt: P09619

Pathways: Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Inositol Metabolic Process, Glycosaminoglycan Metabolic Process, Smooth Muscle Cell Migration, Platelet-derived growth Factor Receptor Signaling