TRF1 Antikörper

Produktnummer ABIN498269

Produktdetails anti-TRF1 Antikörper

Target: TRF1 (TERF1) (Telomeric Repeat Binding Factor (NIMA-Interacting) 1 (TERF1))

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser TRF1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Spezifität: This antibody detects endogenous levels of TRF1 protein.

Kreuzreaktivität (Details): Species reactivity (expected):Mouse.
Species reactivity (tested):Human.

Aufreinigung: Affinity chromatography using epitope-specific immunogen

Reinheit: > 95 % (by SDS-PAGE)

Anwendungsinformationen

Applikationshinweise: Western blot: 1: 500 - 1: 1000. Immunohistochemistry on paraffin sections: 1: 50 - 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS) with 0.05 % sodium azide, approx. pH 7.2

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.

Details zu TRF1

Target: TRF1 (TERF1) (Telomeric Repeat Binding Factor (NIMA-Interacting) 1 (TERF1))

Andere Bezeichnung: TERF1 (TERF1 Produkte)

Synonyme: PIN2 antikoerper, TRBF1 antikoerper, TRF antikoerper, TRF1 antikoerper, hTRF1-AS antikoerper, t-TRF1 antikoerper, wu:fb74c04 antikoerper, TERF1 antikoerper, DKFZp459A0713 antikoerper, Pin2 antikoerper, Trbf1 antikoerper, Trf1 antikoerper, terf1 antikoerper, trf1 antikoerper, telomeric repeat binding factor 1 antikoerper, telomeric repeat binding factor (NIMA-interacting) 1 antikoerper, telomeric repeat binding factor (NIMA-interacting) 1 L homeolog antikoerper, TERF1 antikoerper, Terf1 antikoerper, terf1 antikoerper, Trf1 antikoerper, terf1.L antikoerper

Hintergrund: Telomeric repeat binding factor 1 (TRF1, TERF1, PIN2, TRBF1) and telomeric repeat binding factor 2 (TRF2, TERF2, TRBF2) are present at telomeres throughout the cell cycle, where they regulate telomerase by acting in cis to limit the elongation of individual chromosome ends. Telomerase adds hexameric repeats of 5'-TTAGGG-3' to the ends of chromosomal DNA. This telomerase enzyme plays an influential role in cellular immortalization and cellular senescence. TRF1 negatively regulates telomere elongation, while TRF2 protects the chromosome ends by inhibiting end-to-end fusions. Downregulation of TRF expression in tumor cells may contribute to cell immortalization and malignant progression. TRF1 has an acidic N-terminus while TRF2 has a basic N-terminus. TRF2 localizes in the nucleolus at G0 and S and diffuses out of the nucleolus in G2 phase. During mitosis TRF2 disperses from the condensed chromosomes and returns to the nucleolus at cytokinesis.Synonyms: NIMA-interacting protein 2, PIN2, TRBF1, TRF, TRF1, TTAGGG repeat-binding factor 1, Telomeric protein Pin2/TRF1, Telomeric repeat-binding factor 1

Molekulargewicht: approx. 50 kDa

Gen-ID: 7013

NCBI Accession: NP_003209

UniProt: P54274

Pathways: Zellzyklus, Telomere Maintenance, Hormone Transport, Chromatin Binding, Regulation of Cell Size, Positive Regulation of Endopeptidase Activity