CDC25A Antikörper

Produktnummer ABIN498254

Der Kaninchen Polyklonal anti-CDC25A Antikörper wird verwendet zum Nachweis von CDC25A in Proben von Human, Maus und Ratte. Er wurde validiert für WB.

Kurzübersicht für CDC25A Antikörper (ABIN498254)

Target: CDC25A (Cell Division Cycle 25 Homolog A (S. Pombe) (CDC25A))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CDC25A Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-CDC25A Antikörper

Spezifität: This antibody detects endogenous levels of Cdc25A protein.

Kreuzreaktivität (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Aufreinigung: Affinity chromatography using epitope-specific immunogen

Reinheit: > 95 % (by SDS-PAGE)

Anwendungsinformationen

Applikationshinweise: Western blot: 1: 500 - 1: 1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS) with 0.05 % sodium azide, approx. pH 7.2

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.

Details zu CDC25A

Target: CDC25A (Cell Division Cycle 25 Homolog A (S. Pombe) (CDC25A))

Andere Bezeichnung: CDC25A

Hintergrund: The Cdc2/cyclin B enzyme, involved in regulation of mitosis in eukaryotic cells, is subject to multiple levels of control. Among these, the regulation of the catalytic subunit by tyrosine phosphorylation is the best understood. tyrosine phosphorylation inhibits the Cdc2/cyclin B complex, while tyrosine dephosphorylation, which occurs at the onset of mitosis, directly activates the pre-MPH complex. The Cdc25 gene serves as a rate-limiting mitotic activator, apparently due to its action as the Cdc2 tyrosine phosphatase. In the absence of Cdc25, Cdc2 accumulates in a tyrosine phosphorylated state. In addition, Cdc25 proteins from a variety of species have been shown to share a low degree of sequence similarity with other tyrosine phosphatases. The Cdc25 gene family consists of at least three members that share approximately 40 % identity in their most conserved carboxy terminal sequences.Synonyms: CDC-25A, Cell Division Cycle 25A, Dual specificity phosphatase Cdc25A, M-phase inducer phosphatase 1

Molekulargewicht: approx. 59 kDa

Gen-ID: 993

NCBI Accession: NP_001780

UniProt: P30304

Pathways: Zellzyklus, Mitotic G1-G1/S Phases, M Phase