ATG5 Antikörper (N-Term)

Produktnummer ABIN497966

Der Kaninchen Polyklonal Anti-ATG5-Antikörper wurde für WB, IHC (p) und EIA validiert. Er ist geeignet, ATG5 in Proben von Human, Maus und Ratte zu detektieren.

Kurzübersicht für ATG5 Antikörper (N-Term) (ABIN497966)

Target: ATG5 (ATG5 Autophagy Related 5 (ATG5))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ATG5 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Produktdetails anti-ATG5 Antikörper

Bindungsspezifität: N-Term

Spezifität: Recognizes APG5L/ATG5.

Aufreinigung: Affinity Chromatography purified via peptide column.

Immunogen: A 16 amino acid peptide from near the amino terminus of Human ATG5

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: ELISA. Western Blot: ATG5 antibody can be used for the detection of ATG5 at 1-2 μg/mL. Positive Control. Rat Spleen Tissue Lysate. Immunohistochemistry. Positive Control. Human Spleen Tissue Lysate.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: PBS containing 0.02 % Sodium Azide as preservative.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C. Antibodies should not be exposed to prolonged high temperatures.

Details zu ATG5

Target: ATG5 (ATG5 Autophagy Related 5 (ATG5))

Andere Bezeichnung: APG5L / ATG5

Hintergrund: Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. This process is negatively regulated by TOR (Target of rapamycin) through phosphorylation of autophagy protein APG1. ATG5, another member of the autophagy protein family, forms a conjugate with ATG12, this conjugate has a ubiquitin-protein ligase (E3)-like activity for protein lipidation in autophagy. This conjugate also associates with innate immune response proteins such as RIG-I and VISA (also known as IPS-1), inhibiting type I interferon production and permitting viral replication in host cells. Three isoforms of ATG5 are known to exist, this ATG5 antibody will only detect the longest isoform.Synonyms: APG5-like, ASP, Apoptosis-specific protein, Autophagy protein 5

Gen-ID: 9474

NCBI Accession: NP_004840

UniProt: Q9H1Y0

Pathways: Activation of Innate immune Response, Production of Molecular Mediator of Immune Response, Autophagie