PKM2 Antikörper (AA 476-505)

Produktnummer ABIN3032237

Der Kaninchen Polyklonal Anti-PKM2-Antikörper wurde für WB, IHC, ELISA und IF validiert. Er ist geeignet, PKM2 in Proben von Human, Maus, Ratte und Primate zu detektieren.

Kurzübersicht für PKM2 Antikörper (AA 476-505) (ABIN3032237)

Target: PKM2 (Pyruvate Kinase M2 (PKM2))

Reaktivität: Human, Maus, Ratte, Primate

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PKM2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Produktdetails anti-PKM2 Antikörper

Bindungsspezifität: AA 476-505

Aufreinigung: Purified

Immunogen: A portion of amino acids 476-505 from the human protein was used as the immunogen for this PKM2 antibody.

Isotyp: Ig Fraction

Anwendungsinformationen

Applikationshinweise: Titration of the PKM2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Immunofluorescence: 1:200,Western blot: 1:1000,IHC (Paraffin): 1:50-1:1:100

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: In 1X PBS pH 7.4 with 0.09 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot the PKM2 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Details zu PKM2

Target: PKM2 (Pyruvate Kinase M2 (PKM2))

Andere Bezeichnung: PKM2

Hintergrund: PKM2 is a glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. Stimulates POU5F1-mediated transcriptional activation. Plays a general role in caspase independent cell death of tumor cells. The ratio betwween the highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production. The transition between the 2 forms contributes to the control of glycolysis and is important for tumor cell proliferation and survival. [UniProt]

UniProt: P14618

Pathways: Warburg Effekt