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PCNA Antikörper (AA 231-261)

Name: PCNA Antikörper (AA 231-261)
SKU: ABIN3032321
Price: 628.5 EUR
Availability: InStock

PCNA Reaktivität: Human WB, IHC, ELISA, IF Wirt: Kaninchen Polyclonal unconjugated

Western blot analysis of PCNA antibody and 293 cell lysate (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (2) with the PCNA gene.

Confocal immunofluorescent analysis of PCNA antibody with 293 cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).

Confocal immunofluorescent analysis of PCNA antibody with HeLa cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).

IHC analysis of FFPE human hepatocarcinoma tissue stained with PCNA antibody

IHC

PCNA antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human lung carcinoma.

IHC

Western blot analysis of PCNA antibody and Jurkat, HeLa, 293 lysate

IHC

10 Abbildungen

Produktnummer ABIN3032321

Datenblatt (PDF)

Target Alle PCNA Antikörper anzeigen

PCNA (Proliferating Cell Nuclear Antigen (PCNA))
Bindungsspezifität
16

16

16

15

13

10

8

6

3

3

3

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 231-261
Reaktivität
222

157

156

57

32

28

23

19

19

18

18

16

11

11

7

5

5

4

4

4

4

3

3

3

2

2

2

2

2

1

1

1

1

1
Human
Wirt
142

93

3
Kaninchen
Passende Sekundärantikörper
Klonalität
141

96

1
Polyklonal
Konjugat
119

18

14

9

6

6

5

5

5

5

5

5

5

5

5

5

2

2

1

1

1

1

1

1

1

1

1

1

1

1
Dieser PCNA Antikörper ist unkonjugiert
Applikation
205

68

67

61

59

50

49

40

34

16

15

12

5

4

3

2

2

2

2

2

1

1

1

1

1

1
Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Kreuzreaktivität (Details)

Expected species reactivity: Rat, Bovine, Primate, Hamster

Aufreinigung

Purified

Immunogen

A portion of amino acids 231-261 from the human protein was used as the immunogen for this PCNA antibody.

Isotyp

Ig Fraction

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anti-Proliferating Cell Nuclear Antigen (PCNA) (AA 6-261) antibody
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anti-Proliferating Cell Nuclear Antigen (PCNA) antibody
PCNA Reaktivität: Human WB, IHC, IHC (p) Wirt: Maus Monoclonal 1H10 unconjugated

Applikationshinweise

Titration of the PCNA antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000,IHC (Paraffin): 1:10-1:50,Immunofluorescence: 1:10-1:50

Beschränkungen

Nur für Forschungszwecke einsetzbar
Format

Liquid

Buffer

In 1X PBS pH 7.4 with 0.09 % sodium azide

Konservierungsmittel

Sodium azide

Vorsichtsmaßnahmen

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung

-20 °C

Informationen zur Lagerung

Aliquot the PCNA antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Target

PCNA (Proliferating Cell Nuclear Antigen (PCNA))

Andere Bezeichnung

PCNA (PCNA Produkte)

Synonyme

PCNAR antikoerper, Pcna/cyclin antikoerper, pcna antikoerper, AO090003000846 antikoerper, 53/13 antikoerper, CG9193 antikoerper, DmPCNA antikoerper, Dmel\\CG9193 antikoerper, MUS209 antikoerper, Mus209 antikoerper, PCNA antikoerper, PCNA/mus209 antikoerper, Pcna antikoerper, dPCNA antikoerper, l(2)02448 antikoerper, l(2)56F antikoerper, l(2)56Fa antikoerper, l(2)s1534 antikoerper, cb16 antikoerper, fb36g03 antikoerper, wu:fa28e03 antikoerper, wu:fb36g03 antikoerper, pcna-A antikoerper, proliferating cell nuclear antigen antikoerper, DNA polymerase III sliding clamp antikoerper, Proliferating cell nuclear antigen antikoerper, Proliferating cell nuclear antigen-like antikoerper, proliferating cell nuclear antigen L homeolog antikoerper, proliferating cell nuclear antigen 1 antikoerper, PCNA antikoerper, Pcna antikoerper, pcna antikoerper, MMP_RS08810 antikoerper, ANI_1_512014 antikoerper, AOR_1_1500154 antikoerper, MGC53867 antikoerper, pcna.L antikoerper, pcna1 antikoerper, LOC106420145 antikoerper, pcn1 antikoerper

Hintergrund

PCNA is found in the nucleus and is a cofactor of DNA polymerase delta. This protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway.

UniProt

P12004

Pathways

Telomere Maintenance, DNA Reparatur, Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA, Autophagie