LIN28B Antikörper (AA 95-128)

Produktnummer ABIN3031627

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch LIN28B in WB, ELISA, IHC, FACS und IF. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für LIN28B Antikörper (AA 95-128) (ABIN3031627)

Target: LIN28B (Lin-28 Homolog B (LIN28B))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser LIN28B Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF)

Produktdetails anti-LIN28B Antikörper

Bindungsspezifität: AA 95-128

Kreuzreaktivität (Details): Expected species reactivity: Mouse

Aufreinigung: Antigen affinity purified

Immunogen: A portion of amino acids 95-128 from the human protein was used as the immunogen for this LIN28B antibody.

Isotyp: Ig Fraction

Anwendungsinformationen

Applikationshinweise: Titration of the LIN28B antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000,IHC (Paraffin): 1:10-1:50,Flow Cytometry: 1:10-1:50,Immunofluorescence: 1:10-1:50

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: In 1X PBS, pH 7.4, with 0.09 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot the LIN28B antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Details zu LIN28B

Target: LIN28B (Lin-28 Homolog B (LIN28B))

Andere Bezeichnung: LIN28B

Hintergrund: LIN28B is a suppressor of microRNA (miRNA) biogenesis, including that of let-7 and possibly of miR107, miR-143 and miR-200c. Binds primary let-7 transcripts (pri-let-7), including pri-let-7g and pri-let-7a-1, and sequester them in the nucleolus, away from the microprocessor complex, hence preventing their processing into mature miRNA. Does not act on pri-miR21. The repression of let-7 expression is required for normal development and contributes to maintain the pluripotent state of embryonic stem cells by preventing let-7-mediated differentiation. When overexpressed, recruits ZCCHC11/TUT4 uridylyltransferase to pre-let-7 transcripts, leading to their terminal uridylation and degradation. This activity might not be relevant in vivo, as LIN28B-mediated inhibition of let-7 miRNA maturation appears to be ZCCHC11-independent. Interaction with target pre-miRNAs occurs via an 5'-GGAG-3' motif in the pre-miRNA terminal loop. Mediates MYC-induced let-7 repression (By similarity). [UniProt]

UniProt: Q6ZN17