CDK2 Antikörper (AA 230-260)

Produktnummer ABIN3030609

Dieses Anti-CDK2-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von CDK2 in WB, IHC, ELISA und FACS. Geeignet für Human.

Kurzübersicht für CDK2 Antikörper (AA 230-260) (ABIN3030609)

Target: CDK2 (Cyclin-Dependent Kinase 2 (CDK2))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CDK2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Flow Cytometry (FACS)

Produktdetails anti-CDK2 Antikörper

Bindungsspezifität: AA 230-260

Kreuzreaktivität (Details): Expected species reactivity: Mouse, Rat, Bovine, Hamster, Xenopus

Aufreinigung: Purified

Immunogen: A portion of amino acids 230-260 from the human protein was used as the immunogen for this CDK2 antibody.

Isotyp: Ig Fraction

Anwendungsinformationen

Applikationshinweise: Titration of the CDK2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000,IHC (Paraffin): 1:50-1:100,Flow Cytometry: 1:10-1:50

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: In 1X PBS, pH 7.4, with 0.09 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot the CDK2 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Details zu CDK2

Target: CDK2 (Cyclin-Dependent Kinase 2 (CDK2))

Andere Bezeichnung: CDK2

Hintergrund: CDK2 is a serine/threonine-protein kinase involved in the control of the cell cycle, essential for meiosis, but dispensable for mitosis. Phosphorylates CTNNB1, USP37, p53/TP53, NPM1, CDK7, RB1, BRCA2, MYC, NPAT, EZH2. Interacts with cyclins A, B1, B3, D, or E. Triggers duplication of centrosomes and DNA. Acts at the G1-S transition to promote the E2F transcriptional program and the initiation of DNA synthesis, and modulates G2 progression, controls the timing of entry into mitosis/meiosis by controlling the subsequent activation of cyclin B/CDK1 by phosphorylation, and coordinates the activation of cyclin B/CDK1 at the centrosome and in the nucleus. Crucial role in orchestrating a fine balance between cellular proliferation, cell death, and DNA repair in human embryonic stem cells (hESCs). Activity of CDK2 is maximal during S phase and G2, activated by interaction with cyclin E during the early stages of DNA synthesis to permit G1-S transition, and subsequently activated by cyclin A2 (cyclin A1 in germ cells) during the late stages of DNA replication to drive the transition from S phase to mitosis, the G2 phase. EZH2 phosphorylation promotes H3K27me3 maintenance and epigenetic gene silencing. Phosphorylates CABLES1 (By similarity). [UniProt]

UniProt: P24941

Pathways: PI3K-Akt Signalweg, Zellzyklus, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Synthesis of DNA