GJB1 Antikörper (Cytoplasmic Loop)

Produktnummer ABIN4949578

Produktdetails anti-GJB1 Antikörper

Target: GJB1 (Gap Junction Protein, beta 1, 32kDa (GJB1))

Bindungsspezifität: Cytoplasmic Loop

Reaktivität: Human, Ratte, Maus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser GJB1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))

Aufreinigung: Protein G affinity chromatography

Immunogen: Rat junctional complexes were used as the immunogen for the Connexin 32 antibody. The epitope is known to be located in the cytoplasmic loop (Ref 1).

Klon: M12-13

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the Connexin 32 antibody should be determined by the researcher.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 1-2 μg/mL,WB: 0.5-1 μg/mL,IHC (Frozen): 0.5-1 μg/mL for 30 min at RT

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Konservierungsmittel: Azide free

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store the Connexin 32 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Details zu GJB1

Target: GJB1 (Gap Junction Protein, beta 1, 32kDa (GJB1))

Andere Bezeichnung: Connexin 32 / GJB1 (GJB1 Produkte)

Synonyme: GJIC antikoerper, cmtx antikoerper, cmtx1 antikoerper, connexin-30 antikoerper, connexin32 antikoerper, cx30 antikoerper, cx32 antikoerper, gja1 antikoerper, CX32 antikoerper, GJB1 antikoerper, AI118175 antikoerper, Cnx32 antikoerper, Cx32 antikoerper, Gjb-1 antikoerper, CMTX antikoerper, CMTX1 antikoerper, gjb1-a antikoerper, gap junction protein beta 1 antikoerper, connexin 32 antikoerper, probable serine/threonine-protein kinase CST antikoerper, gap junction protein, beta 1 antikoerper, gap junction protein beta 1 L homeolog antikoerper, gjb1 antikoerper, CX32 antikoerper, LOC9303136 antikoerper, GJB1 antikoerper, Gjb1 antikoerper, gjb1.L antikoerper

Hintergrund: This Ab recognizes a protein of 27-32  kDa, identified as Connexin 32. The connexin family of proteins forms hexameric complexes called connexons that facilitate movement of low molecular weight proteins between cells via gap junctions. Connexin proteins share a common topology of four transmembrane alpha-helical domains, two extracellular loops, a cytoplasmic loop and cytoplasmic N- and C-termini. Many of the key functional differences arise from specific amino-acid substitutions in the most highly conserved domains, the transmembrane and extracellular regions. Each of the approximately 20-connexin isoforms produces channels with distinct permeability and electrical and chemical sensitivities, therefore, one connexin usually cannot fully substitute for another.

Pathways: Cell-Cell Junction Organization