GJB1 Antikörper (Cytoplasmic Loop)

Produktnummer ABIN4949578

Der Maus Monoklonal Anti-GJB1-Antikörper wurde für WB, IF, FACS und IHC (fro) validiert. Er ist geeignet, GJB1 in Proben von Human, Ratte und Maus zu detektieren.

Kurzübersicht für GJB1 Antikörper (Cytoplasmic Loop) (ABIN4949578)

Target: GJB1 (Gap Junction Protein, beta 1, 32kDa (GJB1))

Reaktivität: Human, Ratte, Maus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser GJB1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))

Klon: M12-13

Produktdetails anti-GJB1 Antikörper

Bindungsspezifität: Cytoplasmic Loop

Aufreinigung: Protein G affinity chromatography

Immunogen: Rat junctional complexes were used as the immunogen for the Connexin 32 antibody. The epitope is known to be located in the cytoplasmic loop (Ref 1).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the Connexin 32 antibody should be determined by the researcher.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 1-2 μg/mL,WB: 0.5-1 μg/mL,IHC (Frozen): 0.5-1 μg/mL for 30 min at RT

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Konservierungsmittel: Azide free

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store the Connexin 32 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Details zu GJB1

Target: GJB1 (Gap Junction Protein, beta 1, 32kDa (GJB1))

Andere Bezeichnung: Connexin 32 / GJB1

Hintergrund: This Ab recognizes a protein of 27-32  kDa, identified as Connexin 32. The connexin family of proteins forms hexameric complexes called connexons that facilitate movement of low molecular weight proteins between cells via gap junctions. Connexin proteins share a common topology of four transmembrane alpha-helical domains, two extracellular loops, a cytoplasmic loop and cytoplasmic N- and C-termini. Many of the key functional differences arise from specific amino-acid substitutions in the most highly conserved domains, the transmembrane and extracellular regions. Each of the approximately 20-connexin isoforms produces channels with distinct permeability and electrical and chemical sensitivities, therefore, one connexin usually cannot fully substitute for another.

Pathways: Cell-Cell Junction Organization