GJB1 Antikörper

Produktnummer ABIN4949576

Der Ratte Monoklonal anti-GJB1 Antikörper (Klon R5-21C) (ABIN4949576) detektiert spezifisch GJB1 in WB, FACS, IF und IHC (fro). Dieser Antikörper reagiert spezifisch mit Proben aus Ratte und Maus.

Kurzübersicht für GJB1 Antikörper (ABIN4949576)

Target: GJB1 (Gap Junction Protein, beta 1, 32kDa (GJB1))

Reaktivität: Ratte, Maus

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser GJB1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))

Klon: R5-21C

Produktdetails anti-GJB1 Antikörper

Aufreinigung: Protein G affinity chromatography

Immunogen: Mouse liver DOC-JR-plasma membranes were used as the immunogen for the Connexin 32 antibody.

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the Connexin 32 antibody should be determined by the researcher.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 1-2 μg/mL,WB: 0.5-1 μg/mL,IHC (Frozen): 1-2 μg/mL for 30 min at RT

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Konservierungsmittel: Azide free

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store the Connexin 32 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Details zu GJB1

Target: GJB1 (Gap Junction Protein, beta 1, 32kDa (GJB1))

Andere Bezeichnung: Connexin 32 / GJB1

Hintergrund: This Ab recognizes a protein of 27-32  kDa, identified as Connexin 32. The connexin family of proteins forms hexameric complexes called connexons that facilitate movement of low molecular weight proteins between cells via gap junctions. Connexin proteins share a common topology of four transmembrane alpha-helical domains, two extracellular loops, a cytoplasmic loop and cytoplasmic N- and C-termini. Many of the key functional differences arise from specific amino-acid substitutions in the most highly conserved domains, the transmembrane and extracellular regions. Each of the approximately 20-connexin isoforms produces channels with distinct permeability and electrical and chemical sensitivities, therefore, one connexin usually cannot fully substitute for another.

Pathways: Cell-Cell Junction Organization