Phospholipase C gamma 1 Antikörper (AA 970-990)

Produktnummer ABIN492624

Dieses Anti-Phospholipase C gamma 1-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von Phospholipase C gamma 1 in WB. Geeignet für Human, Maus, Ratte und Hund.

Kurzübersicht für Phospholipase C gamma 1 Antikörper (AA 970-990) (ABIN492624)

Target: Phospholipase C gamma 1 (PLCG1) (phospholipase C, gamma 1 (PLCG1))

Reaktivität: Human, Maus, Ratte, Hund

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Phospholipase C gamma 1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Klon: 2D5

Produktdetails anti-Phospholipase C gamma 1 Antikörper

Bindungsspezifität: AA 970-990

Spezifität: This monoclonal PLCG antibody (Clone 2D5) specifically recognizes Phospholipase C gamma at 150 kDa in Western blot applications.

Produktmerkmale: Synonyms: Phospholipase C gamma, PLC-gamma, Phosphoinositide Phospholipase C, PLC-148, PLC-II,PLC-gamma-1, PLC1, PLCG-1, EC=3.1.4.11, Phospholipase C-gamma-1

Aufreinigung: Subsequent Ultrafiltration and Size Exclusion Chromatography.

Bestandteile: incl. positive Control

Immunogen: Synthetic peptide conjugated to hemocyanin corresponding to amino acids 970-990 ofPLCgamma (Phospholipase C gamma).

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Immunoblotting: 0.5 μg/mL for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer AS00002BU-N or AS00002BU-L. Included Positive Control: Cell lysate from untreated SKOV-3 cells (See Protocols for moredetails).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Protokoll: Positive Control Provided. Cell lysate from untreated SKOV-3 cellsDescription: Cell lysate from untreated SKOV-3 cells, ovary adenocarcinoma (Human)Format: Lyophilized cell lysate from serum starved SKOV-3 cells. Reconstitution: Restore by addition of 200 µl H20. After complete solubilization add 200 µl2x SDS-PAGE sample buffer, mix and incubate at 90°C for 5 min. Storage: Aliquote and store frozen. Avoid repeated freeze/thaw cycles.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Restore with 1 mL H2O (15 min, RT).

Buffer: 1 mLPBS, containing 0.09 % Sodium Azide/PEG and Sucrose.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: For long-term storage, freeze lyophilizate upon arrival (-20 °C). Upon reconstitution, aliquote and freeze in liquid nitrogen, reconstituted antibody can bestored frozen at -80 °C up to 1 year. Avoid repeated freeze / thaw cycles. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 2-8 °C up to 3 months.

Haltbarkeit: 12 months

Details zu Phospholipase C gamma 1

Target: Phospholipase C gamma 1 (PLCG1) (phospholipase C, gamma 1 (PLCG1))

Andere Bezeichnung: PLCG1

Hintergrund: Phospholipase C gamma (PLCG) binds to receptor tyrosine kinases, e.g. EGFR, PDGFR, leading to phosphorylation of PLCgamma at Tyr 771, 783 and 1245. Phosphorylation of Tyr 783 leads to enzymatic activation of PLCgamma. PLC-gamma hydrolyzes phosphatidylinostitol-4,5-bisphosphate (PIP2), thus generating the second messengers inostitol-1,4,5-triphosphate (IP3) and diacylglycerol (DAG).Synonyms: EC=3.1.4.11, PLC-148, PLC-II, PLC-gamma, PLC-gamma-1, PLC1, PLCG-1, Phosphoinositide Phospholipase C, Phospholipase C gamma, Phospholipase C-gamma-1

Gen-ID: 5335

UniProt: P19174

Pathways: RTK Signalweg, WNT Signalweg, T-Zell Rezeptor Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Thyroid Hormone Synthesis, Inositol Metabolic Process, Myometrial Relaxation and Contraction, Regulation of Muscle Cell Differentiation, Regulation of G-Protein Coupled Receptor Protein Signaling, Skeletal Muscle Fiber Development, G-protein mediated Events, Signaling Events mediated by VEGFR1 and VEGFR2, Interaction of EGFR with phospholipase C-gamma, VEGFR1 Specific Signals, VEGF Signaling