ATG5 Antikörper

Produktnummer ABIN492606

Der Maus Monoklonal anti-ATG5 Antikörper (Klon 7C6) (ABIN492606) detektiert spezifisch ATG5 in WB, IF und FACS. Dieser Antikörper reagiert spezifisch mit Proben aus Human, Maus, Ratte und Hund.

Kurzübersicht für ATG5 Antikörper (ABIN492606)

Target: ATG5 (ATG5 Autophagy Related 5 (ATG5))

Reaktivität: Human, Maus, Ratte, Hund

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser ATG5 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS)

Klon: 7C6

Produktdetails anti-ATG5 Antikörper

Spezifität: Recognizes the ATG5-ATG12 protein complex at 55 kDa and ATG5 at 33 kDa in Immunoblot application.

Produktmerkmale: Synonyms: ASP, APG5-like, Autophagy protein 5, Apoptosis-specific protein

Aufreinigung: Purified from Serum-Free Cell Culture Supernatant by subsequent ultrafiltration and Size Exclusion Chromatography

Bestandteile: incl. positive Control

Immunogen: Recombinant ATG5

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Immunobotting (Western Blot): 0.5 μg/mL for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking buffer and blotincubation buffer. Immunocytochemistry: 0.5 μg/mL. Flow Cytometry. Included Postitive Control: Cell lysate from untreated SH-SY5Y cells (See Protocols for moredetails).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Protokoll: Positive Control: Cell lysate from untreated SH-SY5Y cells. Formulation: Lyophilized cell lysate from Serum starved SH-SY5Y cells. Stability: Reconstitute by addition of 200 µl H2O. After complete solubilization add 200 µl 2xSDS-PAGE sample buffer, mix and incubate at 90°C for 5 min. Application: The Positive Control lysate is recommended for Immunoblot applications. 20µl of PositiveControl correspond to ca. 20. 000 cells. Use 20µl/lane (mini gel) for HRPO/ECL detection of the target proteins. Storage: Aliquote and store frozen. Avoid repeated freeze/thaw cycles. Shelf life: one year from despatch. The Lyophilized cell lysates contain SDS and are not recommended for applications withnative proteins such as Immunoprecipitation.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Restore with 1 mL H2O (15 min, RT).

Buffer: PBS, 0.09 % Sodium Azide, PEG and Sucrose

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store lyophilized (preferably in a desiccator) at -20 °C and reconstituted (aliquote andfreeze in liquid nitrogen) at -20 °C to -80 °C. Avoid repeated freezing and thawing. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 2-8 °C up to 3 months.
Shelf life: one year from despatch.

Haltbarkeit: 12 months

Details zu ATG5

Target: ATG5 (ATG5 Autophagy Related 5 (ATG5))

Andere Bezeichnung: APG5L / ATG5

Hintergrund: Autophagy as a response to cellular stress and starvation is an alternative process of proteasomal degradation for some long-lived proteins and organelles. Alterations in the autophagic-lysosomal compartment have been linked to neuronal death in many neurodegenerative disorders as well as in transmissible neuronal pathologies (prion diseases). The gene product of autophagy-related gene 5 (ATG5) is required for autophagosome-formation. ATG5 also enhances the susceptibility towards apoptotic stimuli. Like Bcl-2, ATG5 exhibits a dual function by modulating both autophagy and apoptosis.Synonyms: APG5-like, ASP, Apoptosis-specific protein, Autophagy protein 5

Molekulargewicht: 24, 33, 55 kDa

Gen-ID: 9474

UniProt: Q9H1Y0

Pathways: Activation of Innate immune Response, Production of Molecular Mediator of Immune Response, Autophagie