RAD50 Antikörper (AA 817-872) (Biotin)

Produktnummer ABIN4913429

Der Kaninchen Polyklonal Anti-RAD50-Antikörper wurde für WB, ELISA, IHC (p) und IHC (fro) validiert. Er ist geeignet, RAD50 in Proben von Human und Maus zu detektieren.

Kurzübersicht für RAD50 Antikörper (AA 817-872) (Biotin) (ABIN4913429)

Target: RAD50 (RAD50 Homolog (S. Cerevisiae) (RAD50))

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser RAD50 Antikörper ist konjugiert mit Biotin

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Produktdetails anti-RAD50 Antikörper (Biotin)

Bindungsspezifität: AA 817-872

Kreuzreaktivität: Human, Maus

Homologie: Rat,Cow,Sheep,Horse,Rabbit

Aufreinigung: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human RAD50

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C for 12 months.

Haltbarkeit: 12 months

Details zu RAD50

Target: RAD50 (RAD50 Homolog (S. Cerevisiae) (RAD50))

Andere Bezeichnung: RAD50

Hintergrund:

Synonyms: DNA repair protein RAD50, RAD50, hRAD50

Background: Component of the MRN complex, which plays a central role in double-strand break (DSB) repair, DNA recombination, maintenance of telomere integrity and meiosis. The complex possesses single-strand endonuclease activity and double-strand-specific 3'-5' exonuclease activity, which are provided by MRE11A. RAD50 may be required to bind DNA ends and hold them in close proximity. This could facilitate searches for short or long regions of sequence homology in the recombining DNA templates, and may also stimulate the activity of DNA ligases and/or restrict the nuclease activity of MRE11A to prevent nucleolytic degradation past a given point (PubMed:11741547, PubMed:9590181, PubMed:9705271, PubMed:9651580). The complex may also be required for DNA damage signaling via activation of the ATM kinase (PubMed:15064416). In telomeres the MRN complex may modulate t-loop formation (PubMed:10888888).

Gen-ID: 10111

UniProt: Q92878

Pathways: DNA Reparatur, Protein targeting to Nucleus