BAX Antikörper (AA 30-110)

Produktnummer ABIN4901051

Der Maus Monoklonal Anti-BAX-Antikörper wurde für WB und IHC (p) validiert. Er ist geeignet, BAX in Proben von Human, Maus, Ratte und Huhn zu detektieren.

Kurzübersicht für BAX Antikörper (AA 30-110) (ABIN4901051)

Target: BAX (BCL2-Associated X Protein (BAX))

Reaktivität: Human, Maus, Ratte, Huhn

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser BAX Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon: 6F11

Produktdetails anti-BAX Antikörper

Bindungsspezifität: AA 30-110

Verwendungszweck: Bax is a protein encoded by the BAX gene which is approximately 21,1 kDa. Bax is localised to the mitochondrion membrane and cytoplasm. It is involved in PEDF induced signalling, apoptosis modulation and signalling and the TNFR1 pathway. This protein falls under the BCL2 protein family. It forms a heterodimer with BCL2, and functions as an apoptotic activator. This protein interacts with, and increases the opening of, the mitochondrial voltage-dependent anion channel, which leads to the loss in membrane potential and the release of cytochrome c. Bax is expressed in a wide variety of tissues. Mutations in the BAX gene may result in colorectal cancer and leukaemia. STJ97725 was developed from clone 6F11 and was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen. This antibody detects endogenous Bax protein.

Spezifität: Bax Mouse Monoclonal Antibody (6F11) detects endogenous levels of BAX

Produktmerkmale: Mouse Monoclonal to Bax.

Aufreinigung: The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

Immunogen: synthetic peptide derived from Bax

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: WB 1:1000-2000
IHC 1:100-200

Kommentare:

Expressed in a wide variety of tissues. Isoform Psi is found in glial tumors. Isoform Alpha is expressed in spleen, breast, ovary, testis, colon and brain, and at low levels in skin and lung. Isoform Sigma is expressed in spleen, breast, ovary, testis, lung, colon, brain and at low levels in skin. Isoform Alpha and isoform Sigma are expressed in pro-myelocytic leukemia, histiocytic lymphoma, Burkitt's lymphoma, T-cell lymphoma, lymphoblastic leukemia, breast adenocarcinoma, ovary adenocarcinoma, prostate carcinoma, prostate adenocarcinoma, lung carcinoma, epidermoid carcinoma, small cell lung carcinoma and colon adenocarcinoma cell lines.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freeze/thaw cycles.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C, and avoid repeat freeze-thaw cycles.

Details zu BAX

Target: BAX (BCL2-Associated X Protein (BAX))

Andere Bezeichnung: Bax

Gen-ID: 581

UniProt: Q07812

Pathways: p53 Signalweg, PI3K-Akt Signalweg, Apoptose, Caspase Kaskade in der Apoptose, Positive Regulation of Endopeptidase Activity, Unfolded Protein Response