RECQL2 Antikörper (AA 122-240)

Produktnummer ABIN4886768

Der Kaninchen Polyklonal Anti-RECQL2-Antikörper wurde für WB validiert. Er ist geeignet, RECQL2 in Proben von Human und Ratte zu detektieren.

Kurzübersicht für RECQL2 Antikörper (AA 122-240) (ABIN4886768)

Target: RECQL2 (WRN) (Werner syndrome RecQ like helicase (WRN))

Reaktivität: Human, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser RECQL2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-RECQL2 Antikörper

Bindungsspezifität: AA 122-240

Verwendungszweck: Anti-Werner's syndrome helicase WRN/WRN Antibody Picoband®

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-Werner's syndrome helicase WRN/WRN Antibody Picoband® (ABIN4886768). Tested in WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: E. coli-derived human WRN recombinant protein (Position: Q122-N240). Human WRN shares 84% amino acid (aa) sequence identity with mouse WRN.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.1-0.5 μg/mL, Human, Rat
1. Ding SL, Shen CY (2008). "Model of human aging: recent findings on Werner's and Hutchinson-Gilford progeria syndromes". Clin Interv Aging3 (3): 431-44. 2. Rossi ML, Ghosh AK, Bohr VA (2010). "Roles of Werner syndrome protein in protection of genome integrity". DNA Repair (Amst.) 9 (3): 331-44.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Details zu RECQL2

Target: RECQL2 (WRN) (Werner syndrome RecQ like helicase (WRN))

Andere Bezeichnung: WRN

Hintergrund:

Synonyms: Werner syndrome ATP-dependent helicase,3.6.4.12 ,DNA helicase, RecQ-like type 3,RecQ3,Exonuclease WRN,3.1.-.-,RecQ protein-like 2,WRN,RECQ3, RECQL2,

Tissue Specificity: Widely expressed with higher expression in spleen, kidney and blood. Overexpressed in lesional psoriatic skin. .

Background: Werner syndrome ATP-dependent helicase, also known as DNA helicase, RecQ-like type 3, is an enzyme that in humans is encoded by the WRN gene. This gene encodes a member of the RecQ subfamily and the DEAH (Asp-Glu-Ala-His) subfamily of DNA and RNA helicases. DNA helicases are involved in many aspects of DNA metabolism, including transcription, replication, recombination, and repair. This protein contains a nuclear localization signal in the C-terminus and shows a predominant nucleolar localization. It possesses an intrinsic 3' to 5' DNA helicase activity, and is also a 3' to 5' exonuclease. Based on interactions between this protein and Ku70/80 heterodimer in DNA end processing, this protein may be involved in the repair of double strand DNA breaks. Defects in this gene are the cause of Werner syndrome, an autosomal recessive disorder characterized by premature aging.

Molekulargewicht: 162 kDa, 200 kDa

Gen-ID: 7486

UniProt: Q14191

Pathways: DNA Reparatur