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LDHB Antikörper (AA 237-334)

Dieses Anti-LDHB-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von LDHB in WB, IHC, ICC, IF und FACS. Geeignet für Human, Ratte, Maus und Affe.
Produktnummer ABIN4886658

Kurzübersicht für LDHB Antikörper (AA 237-334) (ABIN4886658)

Target

Alle LDHB Antikörper anzeigen
LDHB (Lactate Dehydrogenase B (LDHB))

Reaktivität

  • 58
  • 29
  • 27
  • 3
  • 3
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
Human, Ratte, Maus, Affe

Wirt

  • 62
  • 22
  • 2
Kaninchen

Klonalität

  • 56
  • 29
Polyklonal

Konjugat

  • 56
  • 14
  • 7
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Dieser LDHB Antikörper ist unkonjugiert

Applikation

  • 69
  • 50
  • 36
  • 29
  • 19
  • 13
  • 10
  • 6
  • 5
  • 5
  • 4
  • 4
  • 4
Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FACS)
  • Bindungsspezifität

    • 33
    • 4
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 237-334

    Verwendungszweck

    Anti-Lactate Dehydrogenase B/LDHB Antibody Picoband®

    Kreuzreaktivität (Details)

    No cross-reactivity with other proteins

    Produktmerkmale

    Anti-Lactate Dehydrogenase B/LDHB Antibody Picoband® (ABIN4886658). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

    Aufreinigung

    Immunogen affinity purified.

    Immunogen

    E. coli-derived human LDHB recombinant protein (Position: E237-L334). Human LDHB shares 98% amino acid (aa) sequence identity with both mouse and rat LDHB.

    Isotyp

    IgG
  • Applikationshinweise

    Western blot, 0.1-0.5 μg/mL, Human, Monkey, Mouse, Rat
    Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
    Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human
    Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human1. Markert, C. L. Lactate dehydrogenase: biochemistry and function of lactate dehydrogenase. Cell Biochem. Func. 2: 131-134, 1984. 2. Okumura, N., Terasawa, F., Ueno, I., Oki, K., Yamauchi, K., Hidaka, H., Tozuka, M., Okura, M., Katsuyama, T. Genetic analyses in homozygous and heterozygous variants of lactate dehydrogenase-B (H) subunit: LD-B Matsumoto I and II (LD-B W323R). Clin. Chim. Acta 287: 163-171, 1999.

    Kommentare

    Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Format

    Lyophilized

    Rekonstitution

    Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

    Konzentration

    500 μg/mL

    Buffer

    Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

    Konservierungsmittel

    Sodium azide

    Vorsichtsmaßnahmen

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Handhabung

    Avoid repeated freezing and thawing.

    Lagerung

    4 °C,-20 °C

    Informationen zur Lagerung

    Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
    It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
  • Target

    LDHB (Lactate Dehydrogenase B (LDHB))

    Andere Bezeichnung

    LDHB

    Hintergrund

    Synonyms: L-lactate dehydrogenase B chain,LDH-B,1.1.1.27,LDH heart subunit,LDH-H,Renal carcinoma antigen NY-REN-46,LDHB,

    Tissue Specificity: Low levels in brain. Very weak expression found in heart and muscle. .

    Background: The LDHB gene encodes the B subunit of lactate dehydrogenase enzyme, which catalyzes the interconversion of pyruvate and lactate with concomitant interconversion of NADH and NAD+ in a post-glycolysis process. Alternatively spliced transcript variants have been found for this gene. Recent studies have shown that a C-terminally extended isoform is produced by use of an alternative in-frame translation termination codon via a stop codon readthrough mechanism, and that this isoform is localized in the peroxisomes. Mutations in this gene are associated with lactate dehydrogenase B deficiency. Pseudogenes have been identified on chromosomes X, 5 and 13.

    Molekulargewicht

    37 kDa

    Gen-ID

    3945

    UniProt

    P07195

    Pathways

    Warburg Effekt
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