EGF Antikörper (Biotin)

Produktnummer ABIN465856

This anti-EGF antibody (ABIN465856) is a Rabbit Polyclonal antibody detecting EGF in WB, ELISA, Neut. Suitable for Human.

Quick Overview for EGF Antikörper (Biotin) (ABIN465856)

Target: EGF (Epidermal Growth Factor (EGF))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser EGF Antikörper ist konjugiert mit Biotin

Applikation: Western Blotting (WB), ELISA, Neutralization (Neut)

Produktdetails anti-EGF Antikörper (Biotin)

Spezifität: Human EGF

Aufreinigung: Immunoaffinity purified

Immunogen: E.coli derived recombinant Human EGF.

Type of Immunogen: Cell extract

Anwendungsinformationen

Applikationshinweise: Approved: ELISA (1 μg/mL), Neut, WB (0.1 - 0.2 μg/mL)

Usage: The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. ELISA: To detect hEGF by direct ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant hEGF. To detect hEGF by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Human EGF (LS-C104654) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hEGF. Western Blot: To detect hEGF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hEGF is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Sterile PBS containing 0.1 % BSA

Konzentration: Lot specific

Buffer: Lyophilized from PBS,  pH 7.2

Handhabung: Avoid freeze/thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Details zu EGF

Target: EGF (Epidermal Growth Factor (EGF))

Andere Bezeichnung: EGF

Hintergrund: Name/Gene ID: EGF

Synonyms: EGF, Beta-urogastrone, HOMG4, URG, Epidermal growth factor, Pro-epidermal growth factor

Gen-ID: 1950

UniProt: P01133

Pathways: NF-kappaB Signalweg, RTK Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation