Leptin Antikörper
-
- Target Alle Leptin (LEP) Antikörper anzeigen
- Leptin (LEP)
-
Reaktivität
- Human
-
Wirt
- Kaninchen
-
Klonalität
- Polyklonal
-
Konjugat
- Dieser Leptin Antikörper ist unkonjugiert
-
Applikation
- Western Blotting (WB), ELISA
- Spezifität
- Human Leptin
- Aufreinigung
- Immunoaffinity purified
- Immunogen
-
E.coli derived recombinant Human Leptin.
Type of Immunogen: Cell extract - Top Product
- Discover our top product LEP Primärantikörper
-
-
- Applikationshinweise
-
Approved: ELISA, WB (0.1 - 0.2 μg/mL)
Usage: Immunohistochemistry: This antibody stained formalin-fixed, paraffin-embedded sections of human breast invasive ductal carcinoma. The recommended concentration is 0.5 μg/mL with an overnight incubation at 4 °C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary. ELISA: To detect hLeptin by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human Leptin as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hLeptin. Western Blot: To detect hLeptin by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hLeptin is 1.5-3 ng/lane, under either reducing or non-reducing conditions. - Kommentare
-
Target Species of Antibody: Human
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
-
- Format
- Lyophilized
- Rekonstitution
- Sterile water
- Konzentration
- Lot specific
- Buffer
- Lyophilized from PBS, pH 7.2
- Handhabung
- Avoid freeze/thaw cycles.
- Lagerung
- 4 °C,-20 °C
- Informationen zur Lagerung
-
Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
-
- Target
- Leptin (LEP)
- Andere Bezeichnung
- Leptin (LEP Produkte)
- Synonyme
- ob antikoerper, obese antikoerper, LEPD antikoerper, OB antikoerper, OBS antikoerper, leptin antikoerper, Lep antikoerper, LEP antikoerper, lep antikoerper
- Hintergrund
-
Name/Gene ID: LEP
Synonyms: LEP, Leptin, LEPD, Obese protein, Obese, mouse, homolog of, Obesity factor, OBS, OB - Gen-ID
- 3952
- UniProt
- P41159
- Pathways
- JAK-STAT Signalweg, AMPK Signaling, Hormone Transport, Peptide Hormone Metabolism, Hormone Activity, Negative Regulation of Hormone Secretion, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Monocarboxylic Acid Catabolic Process
-