TGFB1 Antikörper

Produktnummer ABIN465317

Der Maus Monoklonal anti-TGFB1 Antikörper wird verwendet zum Nachweis von TGFB1 in Proben von Human, Maus und Affe. Er wurde validiert für WB und ELISA.

Kurzübersicht für TGFB1 Antikörper (ABIN465317)

Target: TGFB1 (Transforming Growth Factor, beta 1 (TGFB1))

Reaktivität: Human, Maus, Affe

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser TGFB1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Produktdetails anti-TGFB1 Antikörper

Spezifität: Human TGF-beta1

Aufreinigung: Protein G purified

Immunogen: Hi-5 Insect cell derived recombinant Human TGF-beta1.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Approved: ELISA, WB

Usage: Immunohistochemistry: This antibody stained formalin-fixed paraffin-embedded sections of human pancreas adenocarcinoma tissue. The recommended concentration is 5 μg/mL with an overnight incubation at 4 °C. An HRP-labeled polymer detection system was used with an alcohol-soluble AEC chromogen. Heat induced antigen retrieval with a  pH 6 sodium citrate buffer is recommended. Optimal concentrations and conditions may vary. Tissue samples were provided by the Cooperative Human Tissue Network, which is funded by the National Cancer Institute. *Additional Immunostaining data available. Please contact Tech Support for information. ELISA: In a sandwich ELISA (assuming 100 μL/well), a concentration of 4-8 μg/mL of this antibody will detect at least 100 pg/mL of recombinant Human TGF-beta1 when used in conjunction with compatible secondary reagents. Western Blot: To detect Human TGF-beta1 by Western Blot analysis this antibody can be used at a concentration of 2-4 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant Human TGF-beta1 is 2-4 ng/lane, under non-reducing conditions. In a dot blot, a 1:500 dilution of this antibody exhibited complete cross reactivity with Human TGF-beta2 and TGF-beta3 isotypes. Reduced cross reactivity was shown at a 1:1000 dilution.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Sterile water

Konzentration: Lot specific

Buffer: Lyophilized from PBS

Handhabung: Avoid freeze/thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.

Details zu TGFB1

Target: TGFB1 (Transforming Growth Factor, beta 1 (TGFB1))

Andere Bezeichnung: TGFB1 / TGF Beta 1

Hintergrund: Name/Gene ID: TGFB1
Family: TGF beta

Synonyms: TGFB1, CED, DPD1, Diaphyseal dysplasia 1, LAP, Latency-associated peptide, TGF Beta, TGFbeta, TGF-beta 1 protein, TGF-beta-1, TGFB

Gen-ID: 7040

UniProt: P01137

Pathways: EGFR Signaling Pathway, Dopaminergic Neurogenesis, Cellular Response to Molecule of Bacterial Origin, Glycosaminoglycan Metabolic Process, Regulation of Leukocyte Mediated Immunity, Regulation of Muscle Cell Differentiation, Positive Regulation of Immune Effector Process, Cell-Cell Junction Organization, Production of Molecular Mediator of Immune Response, Ribonucleoside Biosynthetic Process, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Protein targeting to Nucleus, Autophagie, Cancer Immune Checkpoints