GRP94 Antikörper

Produktnummer ABIN452391

Produktdetails anti-GRP94 Antikörper

Target: GRP94 (HSP90B1) (Heat Shock Protein 90kDa beta (Grp94), Member 1 (HSP90B1))

Reaktivität: Human, Maus, Ratte, Rind (Kuh), Huhn, Hamster, Hund, Kaninchen, Schwein, Meerschweinchen, Affe, Schaf, Pferd, Xenopus laevis

Wirt: Ratte

Klonalität: Monoklonal

Konjugat: Dieser GRP94 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Flow Cytometry (FACS), Immunoprecipitation (IP)

Spezifität: This antibody detects a 98 kDa protein corresponding to the molecular mass of Grp94 on SDS PAGE immunoblots. Does not detect human Hsp90, Grp74 or GrpE from E. coli.

Kreuzreaktivität (Details): Species reactivity (tested):Human, mouse, rat, bovine, canine, chicken, guinea pig, hamster, horse, monkey, pig, rabbit, sheep, Xenopus

Produktmerkmale: Synonyms: Tumor rejection antigen 1, GRP94, Heat shock protein 90 kDa beta member 1, 94 kDaglucose-regulated protein

Aufreinigung: Protein G affinity chromatography

Immunogen: Purified Grp94 isolated from chicken oviducts

Klon: 9G10

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: Western blot (8, 9, 10). Immunoprecipitation (11-14). Flow cytometry.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: PBS pH 7.2, 50 % glycerol, 0.09 % azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C. Avoid repeated freezing andthawing.
Shelf life: one year from despatch.

Haltbarkeit: 12 months

Details zu GRP94

Target: GRP94 (HSP90B1) (Heat Shock Protein 90kDa beta (Grp94), Member 1 (HSP90B1))

Andere Bezeichnung: Endoplasmin / HSP90B1 / TRA1 (HSP90B1 Produkte)

Hintergrund: Grp94 (glucose regulated protein 94, gp96) is a constitutively expressed endoplasmic reticulum (ER) lumenal protein that is up-regulated in response to cellular stress such as heat shock, oxidative stress or glucose depletion. Grp94 is thought to play a role in protein translocation to the ER, in their subsequent folding and assembly, and in regulating protein secretion (1). Grp94 also plays a role in antigen presentation by accessing the endogenous pathway and eliciting specific CTL responses to chaperone bound peptides via MHC class I pathway (2). Grp94 is a member of the Hsp90 family of stress proteins and shares sequence homolgy with its cytosolic equivalent, Hsp90 (3). Both Hsp90 and Grp94 are calcium binding proteins (4). Despite sharing 50 % sequence homology over its N domains and complete conservation in its ligand binding domains with Hsp90, Grp94 and Hsp90 differ in their interactions with regulatory ligands as Grp94 has weak ATP binding and hydrolyisis activity (5). Grp94 exists as a homodimer and the two subunits interact at two distinct intermolecular sites, C terminal dimerization domains and the N-terminal interacts with the middle domain of opposing subunits. (6). Grp94 contains a carboxy terminal KDEL (Lys-Asp-Glu-Leu) sequence which is believed to aid in its retention in the ER (7).Synonyms: 94 kDa glucose-regulated protein, GRP94, Heat shock protein 90 kDa beta member 1, Tumor rejection antigen 1

Gen-ID: 7184

UniProt: P14625

Pathways: Thyroid Hormone Synthesis, Activation of Innate immune Response, ER-Nucleus Signaling, Toll-Like Receptors Cascades