Rekombinanter RFP Antikörper (AZDye 568)

Produktnummer ABIN4368331

Der Alpaca Monoklonal Anti-RFP-Antikörper wurde für IF, FACS und ICC validiert. Er ist geeignet, RFP in Proben von Discosoma zu detektieren.

Kurzübersicht für Rekombinanter RFP Antikörper (AZDye 568) (ABIN4368331)

Target: RFP (Red Fluorescent Protein (RFP))

Antikörpertyp: Recombinant Antibody

Fragment: single-domain Antibody (sdAb)

Reaktivität: Discosoma

Wirt: Alpaca

Klonalität: Monoklonal

Konjugat: Dieser RFP Antikörper ist konjugiert mit AZDye 568

Applikation: Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Klon: 2B12

Produktdetails anti-RFP Antikörper (AZDye 568)

Expressionssystem: E.coli

Verwendungszweck: Camelid sdAb anti-RFP conjugated with AZDye 568 (Alexa Fluor 568 equivalent), Clone 2B12

Spezifität: Recognizes most common red fluorescent proteins like mRFP and derivatives like mCherry, mScarlet-i, tdTomato, dsRed and mOrange.

Kreuzreaktivität (Details): Does not cross-react with GFP or mTagBFP derivatives.

Produktmerkmale: A dye conjugated alpaca single-domain antibody (sdAb), also referred to as VHH or nanobody. It is in the range of 15 kDa and 3 nm in molecular weight and size respectively. This means that sdAbs are ten times lighter and up to 5x smaller than a conventional IgG molecule. They can position a fluorophore up to 20 nm closer to the intended target than using conventional primary-secondary antibody complex detection.

Aufreinigung: Produced in: E.coli

Immunogen: Recombinant protein corresponding to AA 1 to 225 from sea anemone RFP (UniProt Id: Q9U6Y8)

Labeling Ratio: a single fluorophore is coupled to exactly one sdAb

Anwendungsinformationen

Applikationshinweise: Recommended dilution 1:500

Kommentare:

Each fluorophore is coupled to exactly one sdAb, which in turn binds to its target molecule in a monovalent fashion. The high binding affinity and a high coupling efficiency of > 95% guarantees a highly linear relation between target molecule number and fluorescent intensity. This enables you to directly count your target molecule of interest. The fluorophore is located exceptionally close to the recognized epitope (< 1.5 nm), which is ideal for all microscopy techniques.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Reconstitute with 50 % glycerol in deionized water. We recommend including 0.1 % sodium azide as a preservative if applicable.

Konzentration: 5 μM

Buffer: lyophilized from PBS pH7.4 with 2% BSA (US-Origin)

Handhabung: Avoid bright light when working with the antibody to minimize photo bleeching of the fluorescent dye.

Lagerung: -20 °C,-80 °C

Informationen zur Lagerung: Up to 3 months store at -20 °C. Up to 12 months store at -80 °C or below

Haltbarkeit: 12 months

Details zu RFP

Target: RFP (Red Fluorescent Protein (RFP))

Andere Bezeichnung: RFP

Molekulargewicht: 26 kDa